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Human EGF Protein, Ultra Low Endotoxin (EGF-HE001-UL) Summary
Product Description |
Recombinant Human EGF Protein is expressed from E.coli without tag.It contains Asn971-Arg1023. |
Product Background |
The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human EGF Protein, Ultra Low Endotoxin (EGF-HE001-UL) Specifications/Details
Protein |
EGF |
Synonyms |
HOMG4; EGF; URG |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asn971-Arg1023 |
Molecular Weight |
The protein has a predicted MW of 5.6 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human EGF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 1.56ug/ml determined by ELISA (QC Test). The affinity constant of 1.33 nM as determined in SPR assay (Biacore T200). |
Product Tag |
No Tag |
Fluorophore |
Unconjugated |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, No Tag with an affinity constant of 1.33 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human EGF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 1.56ug/ml determined by ELISA (QC Test). |
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Human EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50-for this effect is typically 0.02 - 0.2 ng/mL (QC Test). |
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The purity of Human EGF is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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