- Express System: E.coli
- Product Tag: N-His
- Purity: > 85% as determined by Bis-Tris PAGE
- Activity: Immobilized Human EGFL7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-EGFL7 Antibody, hFc Tag with the EC50 of 19.5ng/ml determined by ELISA.
- Amino Acid Range: Tyr24-Ser273
Human EGFL7 Protein (EGF-HE1L7) Summary
Product Description |
Recombinant Human EGFL7 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Tyr24-Ser273. |
Product Background |
Epidermal growth factor-like domain-containing protein 7 (EGFL7), a member of the epidermal growth factor (EGF)-like protein family, is a potent angiogenic factor expressed in many different cell types. EGFL7 plays a vital role in controlling vascular angiogenesis during embryogenesis, organogenesis, and maintaining skeletal homeostasis. |
Human EGFL7 Protein (EGF-HE1L7) Specifications/Details
Protein |
EGFL7 |
Synonyms |
EGF-like protein 7; NOTCH4-like protein; VE-statin; NEU1; ZNEU1; EGFL7; MEGF7; MGC111117; RP11-251M1.2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Tyr24-Ser273 |
Molecular Weight |
The protein has a predicted MW of 28.4 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human EGFL7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-EGFL7 Antibody, hFc Tag with the EC50 of 19.5ng/ml determined by ELISA. |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
E.coli |
Purity |
> 85% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in 4mM HCl. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Other Available Proteins: