Name: Human EGFR/HER1 Protein (EGF-HM101)
Brand: KactusBio
SKU: EGF-HM101-100UG
Price: 315.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human EGFR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA.
Amino Acid Range: Leu25-Ser645

Catalog #EGF-HM101

Size

100UG

500UG

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Human EGFR/HER1 Protein (EGF-HM101) Summary

Product Description	Recombinant Human EGFR/HER1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu25-Ser645.
Product Background	The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets \| ADC targets

Human EGFR/HER1 Protein (EGF-HM101) Specifications/Details

Protein	EGFR/HER1
Synonyms	ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Accession	P00533-1
Species	Human
Biotinylated	no
Amino Acid Range	Leu25-Ser645
Molecular Weight	The protein has a predicted MW of 70.19 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human EGFR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human EGFR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA.
	Human EGFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human EGF, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.25 µM as determined in BLI assay .
	Loaded Human TGF-alpha, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.27 µM as determined in BLI assay .
	The purity of Human EGFR is greater than 95% as determined by SEC-HPLC.

Other Available Proteins:

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