Name: Human EGFRVIII Protein (EG8-HM103)
Brand: KactusBio
SKU: EG8-HM103-100UG
Price: 315.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human EGFRVIII, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 43.3ng/ml determined by ELISA.
Amino Acid Range: Leu25-Ser378

Catalog #EG8-HM103

Size

100UG

500UG

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Human EGFRVIII Protein (EG8-HM103) Summary

Product Description	Recombinant Human EGFRVIII Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu25-Ser378.
Product Background	The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets, ADC targets

Human EGFRVIII Protein (EG8-HM103) Specifications/Details

Protein	EGFRVIII
Synonyms	ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Accession	NP_001333870.1
Species	Human
Biotinylated	no
Amino Acid Range	Leu25-Ser378
Molecular Weight	The protein has a predicted MW of 40.25 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human EGFRVIII, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 43.3ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human EGFRVIII, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 43.3ng/ml determined by ELISA.
	Human EGFRVIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human EGFRVIII is greater than 95% as determined by SEC-HPLC.

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