Name: Human EGFRVIII Protein (EG8-HM254)
Brand: KactusBio
SKU: EG8-HM254-100UG
Price: 330.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human EGFR VIII, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EGFRVIII Antibody, hFc Avi Tag with the EC50 of 23.0ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Leu25-Ser378

Catalog #EG8-HM254

Size

100UG

500UG

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Product Description	Recombinant Human EGFRVIII Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Leu25-Ser378.
Product Background	The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	EGFRVIII
Synonyms	ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Accession	NP_001333870.1
Species	Human
Biotinylated	no
Amino Acid Range	Leu25-Ser378
Molecular Weight	The protein has a predicted MW of 64.58 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human EGFR VIII, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EGFRVIII Antibody, hFc Avi Tag with the EC50 of 23.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human EGFR VIII, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EGFRVIII Antibody, hFc Avi Tag with the EC50 of 23.0ng/ml determined by ELISA.
	Human EGFRVIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human EGFRVIII is greater than 95% as determined by SEC-HPLC.