Human EGFRVIII Protein (EG8-HM254)

Immobilized Human EGFR VIII, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EGFRVIII Antibody, hFc Avi Tag with the EC50 of 23.0ng/ml determined by ELISA.

Human EGFRVIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human EGFRVIII is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human EGFR VIII, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EGFRVIII Antibody, hFc Avi Tag with the EC50 of 23.0ng/ml determined by ELISA.
Amino Acid Range: Leu25-Ser378

Catalog #EG8-HM254

Price

$330⁰⁰

Size

Order by 4pm ET for delivery on:

Human EGFRVIII Protein (EG8-HM254) Summary

Product Description

Recombinant Human EGFRVIII Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Leu25-Ser378.

Product Background

The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.

Human EGFRVIII Protein (EG8-HM254) Specifications/Details

Protein	EGFRVIII
Synonyms	ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2;EC2.7.10; EC2.7.10.1
Accession	NP_001333870.1
Species	Human
Biotinylated	no
Amino Acid Range	Leu25-Ser378
Molecular Weight	The protein has a predicted MW of 64.58 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human EGFR VIII, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EGFRVIII Antibody, hFc Avi Tag with the EC50 of 23.0ng/ml determined by ELISA.
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human EGFR VIII, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EGFRVIII Antibody, hFc Avi Tag with the EC50 of 23.0ng/ml determined by ELISA.
	Human EGFRVIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human EGFRVIII is greater than 95% as determined by SEC-HPLC.