Human EMMPRIN/CD147 Protein (EPN-HM147)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human EMMPRIN, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EMMPRIN Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala22-His205

Catalog: EPN-HM147

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Product Description	Recombinant Human EMMPRIN/CD147 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala22-His205.
Product Background	CD147, also known as extracellular matrix metalloproteinase inducer (EMMPRIN) or basigin, is expressed in a variety of cell types. It is involved in the regulation of extracellular matrix (ECM) remodeling during physiological and pathological processes including wound healing, inflammatory diseases, and cancer. CD147 is a diagnostic and therapeutic target in cancer and inflammatory diseases, either directly or indirectly, by targeting CD147 partners.
Product Category	Recombinant Protein

Target	EMMPRIN/CD147
Synonyms	Basigin; BSG; CD147; 5F7; M6; OK; TCSF
Gene/Protein ID	NP_940991.1
Species	Human
Exact Sequence	Ala22-His205
Molecular Weight	The protein has a predicted MW of 21.2 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human EMMPRIN, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EMMPRIN Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human EMMPRIN, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EMMPRIN Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA.
	The purity of Human EMMPRIN is greater than 95% as determined by SEC-HPLC.
	Human EMMPRIN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.