Human Endoglin/CD105 Protein (EDG-HM401)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human Endoglin at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 19.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu26-Gly586

Catalog: EDG-HM401

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Product Description Recombinant Human Endoglin/CD105 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Glu26-Gly586.
Product Category Recombinant Protein
Target Endoglin/CD105
Synonyms CD105; Endoglin; ENG; ORW; ORW1; END; HHT1
Gene/Protein ID P17813-1
Species Human
Exact Sequence Glu26-Gly586
Molecular Weight The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 70-82 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Endoglin at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 19.4ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Endoglin at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 19.4ng/ml determined by ELISA.
The purity of Human Endoglin is greater than 95% as determined by SEC-HPLC.
Human Endoglin on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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