Product Description |
Recombinant Human Endoglin/CD105 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu26-Gly586. |
Product Background |
Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins. Endoglin is a vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis. Required for normal structure and integrity of adult vasculature (PubMed:7894484). Regulates the migration of vascular endothelial cells. Required for normal extraembryonic angiogenesis and for embryonic heart development. May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis. |
Product Category |
Recombinant Protein |
Target |
Endoglin/CD105 |
Synonyms |
CD105; Endoglin; ENG; ORW; ORW1; END; HHT1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu26-Gly586 |
Molecular Weight |
The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 70-82 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Endoglin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Human Endoglin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. |
|
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Human Endoglin on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of Human Endoglin is greater than 95% as determined by SEC-HPLC. |
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