Product Description |
Recombinant Human ENPP-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys98-Asp925. |
Product Background |
Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression. |
Product Category |
Recombinant Protein |
Target |
ENPP-1 |
Synonyms |
E-NPP 1; NPPase; ENPP1; NPPS; PC1; PDNP1; Ly-41 Antigen; M6S1; M6S1NPP1; NPP1; PCA1; PCA1ARHR2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Lys98-Asp925 |
Molecular Weight |
The protein has a predicted MW of 96.5 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA. |
|
|
Human ENPP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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