Human ENPP-1 Protein (ENP-HM102)
Human ENPP-1 Protein (ENP-HM102)
Human ENPP-1 Protein (ENP-HM102)
Human ENPP-1 Protein (ENP-HM102)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Lys98-Asp925
Catalog #ENP-HM102
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Product Description
 Recombinant Human ENPP-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys98-Asp925.
Product Background
 Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression.
Product Category
 Recombinant Protein
Target
 ENPP-1
Synonyms
 E-NPP 1; NPPase; ENPP1; NPPS; PC1; PDNP1; Ly-41 Antigen; M6S1; M6S1NPP1; NPP1; PCA1; PCA1ARHR2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Lys98-Asp925
Molecular Weight
 The protein has a predicted MW of 96.5 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA.
Human ENPP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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