Human ENPP-1 Protein (ENP-HM103)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 17.7ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Lys98-Asp925

Catalog: ENP-HM103

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human ENPP-1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Lys98-Asp925.
Product Background	Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression.
Product Category	Recombinant Protein

Target	ENPP-1
Synonyms	E-NPP 1; NPPase; ENPP1; NPPS; PC1; PDNP1; Ly-41 Antigen; M6S1; M6S1NPP1; NPP1; PCA1; PCA1ARHR2
Accession	P22413
Species	Human
Amino Acid Range	Lys98-Asp925
Molecular Weight	The protein has a predicted MW of 96.5 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 17.7ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 17.7ng/ml determined by ELISA.
	Human ENPP-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.