Human ENPP-1 Protein (ENP-HM103)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 17.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Lys98-Asp925

Catalog: ENP-HM103

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Product Description Recombinant Human ENPP-1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Lys98-Asp925.

Product Background

Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression.

Product Category

Recombinant Protein

Target

ENPP-1
Synonyms E-NPP 1; NPPase; ENPP1; NPPS; PC1; PDNP1; Ly-41 Antigen; M6S1; M6S1NPP1; NPP1; PCA1; PCA1ARHR2

Gene/Protein ID

P22413

Species

Human

Exact Sequence

Lys98-Asp925
Molecular Weight The protein has a predicted MW of 96.5 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 17.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ENPP-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 17.7ng/ml determined by ELISA.
Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is > 40000 pmol/min/ug, as measured under the described conditions.
Human ENPP-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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