Human ENPP-1 Protein, Ultra Low Endotoxin (ENP-HM103-UL)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human ENPP-1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Lys98-Asp925
Catalog #ENP-HM103-UL
Regular price $399.00
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Product Description

Recombinant Human ENPP-1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Lys98-Asp925.

Product Background

Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

ENPP-1

Synonyms

E-NPP 1; NPPase; ENPP1; NPPS; PC1; PDNP1; Ly-41 Antigen; M6S1; M6S1NPP1; NPP1; PCA1; PCA1ARHR2

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Lys98-Asp925

Molecular Weight

The protein has a predicted MW of 96.5 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human ENPP-1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 0.01 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human ENPP-1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA. Immobilized Human ENPP-1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA.
Human ENPP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human ENPP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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