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Human ENPP-1 Protein, Ultra Low Endotoxin (ENP-HM103-UL) Summary
Product Description |
Recombinant Human ENPP-1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Lys98-Asp925. |
Product Background |
Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human ENPP-1 Protein, Ultra Low Endotoxin (ENP-HM103-UL) Specifications/Details
Protein |
ENPP-1 |
Synonyms |
E-NPP 1; NPPase; ENPP1; NPPS; PC1; PDNP1; Ly-41 Antigen; M6S1; M6S1NPP1; NPP1; PCA1; PCA1ARHR2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Lys98-Asp925 |
Molecular Weight |
The protein has a predicted MW of 96.5 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human ENPP-1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA. See testing image for detail.Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is > 40000 pmol/min/ug. |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human ENPP-1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA. |
|
|
Human ENPP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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