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Human ENPP-2/Autotaxin Protein, Ultra Low Endotoxin (ENP-HM101-UL) Summary
Product Description |
Recombinant Human ENPP-2/Autotaxin Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Asp49-Ile863. |
Product Background |
Ectonucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2) also known as Autotaxin, is a secreted lysophospholipase D, which hydrolyzes lysophosphatidylcholine (LPC) into Lysophosphatidic acid (LPA). ENPP2 is an essential protein for normal development and its altered expression is associated with various human diseases. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human ENPP-2/Autotaxin Protein, Ultra Low Endotoxin (ENP-HM101-UL) Specifications/Details
Protein |
ENPP-2/Autotaxin |
Synonyms |
PDNP2; ATX-X; NPP2; PD-IALPHA; ENPP-2; E-NPP 2; Autotaxin; LysoPLD; ATX |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp49-Ile863 |
Molecular Weight |
The protein has a predicted MW of 94.8 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Bis-Tris PAGE result. |
Activity |
Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is > 10000 pmol/min/ug. |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human ENPP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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