Human ENPP-3 Protein (ENP-HM213)
Human ENPP-3 Protein (ENP-HM213)
  • Express System: HEK293
  • Product Tag: N-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human ENPP-3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-ENPP3 Antibody, hFc Avi Tag with the EC50 of 77.2ng/ml determined by ELISA. See testing image for detail.Measured-by-its-ability-to-hydrolyze-thymidine-5'-monophosphate p-nitrophenyl-ester.-The-specific-activity-is->-6000-pmol/min/ug.
  • Amino Acid Range: Leu48-Ile875
Catalog #ENP-HM213
Regular price $363.00
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Size
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Human ENPP-3 Protein (ENP-HM213) Summary

Product Description

Recombinant Human ENPP-3 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-terminus.It contains Leu48-Ile875.

Product Background

Ectonucleotide pyrophosphatase-phosphodiesterase 3 (ENPP3), a protein detected in the human uterus, has been found to play an important role in the development and invasion of tumours. It was recently discovered that ENPP3 was upregulated during the window of implantation in the human endometrium but its functional relevance remains elusive.

Product Category

Recombinant Protein / Antibody discovery / ADC targets

Human ENPP-3 Protein (ENP-HM213) Specifications/Details

Protein
ENPP-3
Synonyms
E-NPP 3; NPP3; PD-Ibeta; NPPase; ENPP3; PDNP3; CD203c; B10; gp130RB13-6; PD-Iβ
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Leu48-Ile875
Molecular Weight
The protein has a predicted MW of 120.58 kDa. Due to glycosylation, the protein migrates to 130-150 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human ENPP-3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-ENPP3 Antibody, hFc Avi Tag with the EC50 of 77.2ng/ml determined by ELISA. See testing image for detail.Measured-by-its-ability-to-hydrolyze-thymidine-5'-monophosphate p-nitrophenyl-ester.-The-specific-activity-is->-6000-pmol/min/ug.
Product Tag
N-hFc (IgG1)

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human ENPP-3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-ENPP3 Antibody, hFc Avi Tag with the EC50 of 77.2ng/ml determined by ELISA. Immobilized Human ENPP-3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-ENPP3 Antibody, hFc Avi Tag with the EC50 of 77.2ng/ml determined by ELISA.
Human ENPP-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human ENPP-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ENPP-3 is greater than 90% as determined by SEC-HPLC. The purity of Human ENPP-3 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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