- Express System: HEK293
- Product Tag: N-hFc (IgG1)
- Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
- Activity: Immobilized Human ENPP-3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-ENPP3 Antibody, hFc Avi Tag with the EC50 of 77.2ng/ml determined by ELISA. See testing image for detail.Measured-by-its-ability-to-hydrolyze-thymidine-5'-monophosphate p-nitrophenyl-ester.-The-specific-activity-is->-6000-pmol/min/ug.
- Amino Acid Range: Leu48-Ile875
Human ENPP-3 Protein (ENP-HM213) Summary
Product Description |
Recombinant Human ENPP-3 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-terminus.It contains Leu48-Ile875. |
Product Background |
Ectonucleotide pyrophosphatase-phosphodiesterase 3 (ENPP3), a protein detected in the human uterus, has been found to play an important role in the development and invasion of tumours. It was recently discovered that ENPP3 was upregulated during the window of implantation in the human endometrium but its functional relevance remains elusive. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Human ENPP-3 Protein (ENP-HM213) Specifications/Details
Protein |
ENPP-3 |
Synonyms |
E-NPP 3; NPP3; PD-Ibeta; NPPase; ENPP3; PDNP3; CD203c; B10; gp130RB13-6; PD-Iβ |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu48-Ile875 |
Molecular Weight |
The protein has a predicted MW of 120.58 kDa. Due to glycosylation, the protein migrates to 130-150 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human ENPP-3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-ENPP3 Antibody, hFc Avi Tag with the EC50 of 77.2ng/ml determined by ELISA. See testing image for detail.Measured-by-its-ability-to-hydrolyze-thymidine-5'-monophosphate p-nitrophenyl-ester.-The-specific-activity-is->-6000-pmol/min/ug. |
Product Tag |
N-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
Other Available Proteins:
Finding available proteins...
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