Name: Human EpCAM/TROP1 Protein, Ultra Low Endotoxin (CAM-HM2EP-UL)
Brand: KactusBio
SKU: CAM-HM2EP-UL-100UG
Price: 236.0 USD
Availability: InStock

Human EpCAM/TROP1 Protein, Ultra Low Endotoxin (CAM-HM2EP-UL)

Human EpCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human AGR-2, His Tag with an affinity constant of 6.91 uM as determined in SPR assay (Biacore T200).

Immobilized Human EpCAM, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 27.4ng/ml determined by ELISA (QC Test).

Human EpCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..

The purity of Human EpCAM is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human EpCAM, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 27.4ng/ml determined by ELISA (QC Test). The affinity constant of 6.91 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln24-Lys265

Catalog #CAM-HM2EP-UL

Size

100UG

500UG

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Product Description	Recombinant Human EpCAM/TROP1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln24-Lys265.
Product Background	Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	EpCAM/TROP1
Synonyms	CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; ACSTD1; DIAR5; gp40; HNPCC8; MOC31; TACST-1; TACSTD1; TROP1; ESA; KSA; MIC18; MK1
Accession	P16422
Species	Human
Biotinylated	no
Amino Acid Range	Gln24-Lys265
Molecular Weight	The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human EpCAM, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 27.4ng/ml determined by ELISA (QC Test). The affinity constant of 6.91 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.001 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human EpCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human AGR-2, His Tag with an affinity constant of 6.91 uM as determined in SPR assay (Biacore T200).
	Immobilized Human EpCAM, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 27.4ng/ml determined by ELISA (QC Test).
	Human EpCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..
	The purity of Human EpCAM is greater than 95% as determined by SEC-HPLC.