Product Description |
Recombinant Human EPHA10 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu34-Ala565. |
Product Background |
EphA10 (erythropoietin-producing hepatocellular carcinoma receptor A10) is a catalytically defective receptor protein tyrosine kinase in the ephrin receptor family.EphA10 was elevated and higher in tumor tissues than in normal tissues in some cancer types, including pancreatic cancer.EphA10 silencing reduced the proliferation, migration, and adhesion of MIA PaCa-2 and AsPC-1 pancreatic cancer cells. EphA10 plays a pivotal role in the tumorigenesis of pancreatic epithelial cells and is a novel therapeutic target for pancreatic cancer. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
EPHA10 |
Synonyms |
EphA10; FLJ16103; FLJ33655; MGC43817 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu34-Ala565 |
Molecular Weight |
The protein has a predicted MW of 58.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human EPHA10, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus EFNA3, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
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Immobilized Human EPHA10, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus EFNA3, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test). |
|
|
Human EPHA10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human EPHA10 is greater than 95% as determined by SEC-HPLC. |
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