HPLC chromatogram showing purity analysis of recombinant protein with a sharp peak at retention time 8.119 minutes.
Human EPHA5 Protein (EPH-HM1A5)
HPLC chromatogram showing purity analysis of recombinant protein with a sharp peak at retention time 8.119 minutes.
Human EPHA5 Protein (EPH-HM1A5)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Ala26-Pro573
Catalog #EPH-HM1A5
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Size
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Human EPHA5 Protein (EPH-HM1A5) Summary

Product Description

 Recombinant Human EPHA5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala26-Pro573.

Product Background

 Ephrin type-A receptor 5 (EphA5) expression was detected in all non-tumor gastric epithelia but was differentially expressed among gastric cancer samples. EphA5 is differentially expressed in gastric cancer. EphA5 may therefore be a potential therapeutic target and may have clinical utility as a marker for lymph node metastasis in gastric cancer.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human EPHA5 Protein (EPH-HM1A5) Specifications/Details

Protein
 EPHA5
Synonyms
 EHK-1; EK7; BSK; EHK1; HEK7; TYRO4; EphA5; Rek7; TYRO4HEK7CEK7
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala26-Pro573
Molecular Weight
 The protein has a predicted MW of 61.9 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human EPHA5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human EPHA5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human EPHA5, His Tag on Anti-His-Biosensor can bind Anti-EPHA5 Antibody, hFc Tag with an affinity constant of 0.63 µM as determined in BLI assay . Loaded Human EPHA5, His Tag on Anti-His-Biosensor can bind Anti-EPHA5 Antibody, hFc Tag with an affinity constant of 0.63 µM as determined in BLI assay .
The purity of Human EPHA5 is greater than 95% as determined by SEC-HPLC. The purity of Human EPHA5 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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