• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.14 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Pro25-Pro573
Catalog #EPH-HM2A5
Price
Regular price $000
Regular price Sale price $000
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Size
Avantor VWR Fisher Scientific

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Human EPHA5 Protein (EPH-HM2A5) Summary

Product Description

 Recombinant Human EPHA5 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Pro25-Pro573.

Product Background

 Ephrin type-A receptor 5 (EphA5) expression was detected in all non-tumor gastric epithelia but was differentially expressed among gastric cancer samples. EphA5 is differentially expressed in gastric cancer. EphA5 may therefore be a potential therapeutic target and may have clinical utility as a marker for lymph node metastasis in gastric cancer.

Human EPHA5 Protein (EPH-HM2A5) Specifications/Details

Protein
 EPHA5
Synonyms
 EHK-1; EK7; BSK; EHK1; HEK7; TYRO4; EphA5; Rek7; TYRO4HEK7CEK7
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Pro25-Pro573
Molecular Weight
 The protein has a predicted MW of 86.74 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 0.14 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human EPHA5, hFc Tag captured on CM5 Chip via ProteinA can bind Human/Cynomolgus/Rhesus macaque Ephrin-A3, His Tag (Cat. EFN-CM1A3) with an affinity constant of 0.14 uM as determined in SPR assay (Biacore T200). Human EPHA5, hFc Tag captured on CM5 Chip via ProteinA can bind Human/Cynomolgus/Rhesus macaque Ephrin-A3, His Tag (Cat. EFN-CM1A3) with an affinity constant of 0.14 uM as determined in SPR assay (Biacore T200).
Human EPHA5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human EPHA5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human EPHA5 is greater than 95% as determined by SEC-HPLC. The purity of Human EPHA5 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...