Human EphA7 Protein (EPH-HM1A7)
Human EphA7 Protein (EPH-HM1A7)
Human EphA7 Protein (EPH-HM1A7)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human EphA7, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.113 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gln28-Val555
  • Other Available Proteins:
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Catalog #EPH-HM1A7
Regular price $330.00
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Human EphA7 Protein (EPH-HM1A7) Summary

Product Description

Recombinant Human EphA7 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln28-Val555.

Product Background

Ephrin receptor A7 (EphA7) is a member of the Eph receptor family. It is widely involved in signal transduction between cells, regulates cell proliferation and differentiation, and participates in developing neural tubes and brain. In addition, EphA7 also has a dual role of tumor promoter and tumor suppressor.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human EphA7 Protein (EPH-HM1A7) Specifications/Details

Protein
EphA7
Synonyms
EHK-3; EHK3; EK11; hEK11; EPHA7;
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln28-Val555
Molecular Weight
The protein has a predicted MW of 60.13 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human EphA7, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.113 uM as determined in SPR assay (Biacore T200).
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human EphA7, His Tag immobilized on CM5 Chip can bind Human Ephrin-A4, His Tag with an affinity constant of 0.113 uM as determined in SPR assay (Biacore T200). Human EphA7, His Tag immobilized on CM5 Chip can bind Human Ephrin-A4, His Tag with an affinity constant of 0.113 uM as determined in SPR assay (Biacore T200).
Immobilized Human EphA7, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). Immobilized Human EphA7, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).
Human EphA7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human EphA7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human EphA7 is greater than 95% as determined by SEC-HPLC. The purity of Human EphA7 is greater than 95% as determined by SEC-HPLC.
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