- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human EphA7, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.113 uM as determined in SPR assay (Biacore T200).
- Amino Acid Range: Gln28-Val555
-
Other Available Proteins:
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Human EphA7 Protein (EPH-HM1A7) Summary
Product Description |
Recombinant Human EphA7 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln28-Val555. |
Product Background |
Ephrin receptor A7 (EphA7) is a member of the Eph receptor family. It is widely involved in signal transduction between cells, regulates cell proliferation and differentiation, and participates in developing neural tubes and brain. In addition, EphA7 also has a dual role of tumor promoter and tumor suppressor. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human EphA7 Protein (EPH-HM1A7) Specifications/Details
Protein |
EphA7 |
Synonyms |
EHK-3; EHK3; EK11; hEK11; EPHA7; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln28-Val555 |
Molecular Weight |
The protein has a predicted MW of 60.13 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human EphA7, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.113 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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