Human EphA7 Protein (EPH-HM1A7)
Human EphA7 Protein (EPH-HM1A7)
Human EphA7 Protein (EPH-HM1A7)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human EphA7, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.113 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln28-Val555
Catalog #EPH-HM1A7
Regular price $300.00
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Product Description
Recombinant Human EphA7 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln28-Val555.
Product Background
Ephrin receptor A7 (EphA7) is a member of the Eph receptor family. It is widely involved in signal transduction between cells, regulates cell proliferation and differentiation, and participates in developing neural tubes and brain. In addition, EphA7 also has a dual role of tumor promoter and tumor suppressor.
Product Category
Recombinant Protein
Target
EphA7
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln28-Val555
Molecular Weight
The protein has a predicted MW of 60.13 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human EphA7, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.113 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human EphA7, His Tag immobilized on CM5 Chip can bind Human Ephrin-A4, His Tag with an affinity constant of 0.113 uM as determined in SPR assay (Biacore T200).
Immobilized Human EphA7, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA (QC Test).
Human EphA7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human EphA7 is greater than 95% as determined by SEC-HPLC.

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