Product Description |
Recombinant Human EphA7 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln28-Val555. |
Product Background |
Ephrin receptor A7 (EphA7) is a member of the Eph receptor family. It is widely involved in signal transduction between cells, regulates cell proliferation and differentiation, and participates in developing neural tubes and brain. In addition, EphA7 also has a dual role of tumor promoter and tumor suppressor. |
Product Category |
Recombinant Protein |
Target |
EphA7 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln28-Val555 |
Molecular Weight |
The protein has a predicted MW of 60.13 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human EphA7, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.113 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human EphA7, His Tag immobilized on CM5 Chip can bind Human Ephrin-A4, His Tag with an affinity constant of 0.113 uM as determined in SPR assay (Biacore T200). |
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|
Immobilized Human EphA7, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA (QC Test). |
|
|
Human EphA7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human EphA7 is greater than 95% as determined by SEC-HPLC. |
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