Product Description |
Recombinant Human EPHB2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val19-Leu543. |
Product Background |
EphB2, a receptor tyrosine kinase for ephrin ligands, is overexpressed in various cancers and plays an important role in tumor progression. EPHB2 promotes endothelial-mesenchymal transition (EMT) and elicits associated pathologic characteristics of glioblastoma multiforme (GBM) such as invasion and migration. EPHB2 is epigenetically overexpressed in hypoxia, a condition highly prevalent in malignancy. Furthermore, HIF-2α is required for EPHB2 stabilization by hypoxia. |
Product Category |
Recombinant Protein |
Target |
EPHB2 |
Synonyms |
DRT; ERK; HEK5; TYRO5; CAPB; EK5; EPHT3; PCBC; Cek5; EphB2; EPHT3MGC87492; Nuk; Qek2; Sek3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val19-Leu543 |
Molecular Weight |
The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human EPHB2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. The affinity constant of 82.97 pM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human Ephrin-B2, hFc Tag captured on CM5 Chip via Protein A can bind Human EPHB2, His Tag with an affinity constant of 82.97 pM as determined in SPR assay (Biacore T200). |
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Immobilized Human EPHB2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. |
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Immobilized Human EPHB2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA (QC Test). |
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Human EPHB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human EPHB2 is greater than 95% as determined by SEC-HPLC. |
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