Name: Human EphB3 Protein (EPH-HM1B3)
Brand: KactusBio
SKU: EPH-HM1B3-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human EphB3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EphB3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
Amino Acid Range: Gly34-Leu559

Catalog #EPH-HM1B3

Size

100UG

500UG

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Human EphB3 Protein (EPH-HM1B3) Summary

Product Description	Recombinant Human EphB3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly34-Leu559.
Product Background	Although EphB3 expression is down-regulated in colorectal cancer (CRC) cells compared with normal intestinal epithelial cells.EphB3 is down-regulated in CRC compared to normal mucosa. Hypermethylation of CpG island is contributed to downregulation of EphB3 in CRC. EphB3 expression in tumor cells may be a useful prognostic indicator for patients with CRC.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human EphB3 Protein (EPH-HM1B3) Specifications/Details

Protein	EphB3
Synonyms	EK2; ETK2; HEK2; TYRO6; EPHB3; AW456895; Cek10; MDK5; Sek4; EC 2.7.10; EC 2.7.10.1
Accession	P54753
Species	Human
Biotinylated	no
Amino Acid Range	Gly34-Leu559
Molecular Weight	The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human EphB3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EphB3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human EphB3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EphB3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
	Human EphB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human EphB3 is greater than 95% as determined by SEC-HPLC.

Other Available Proteins:

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