Product Description |
Recombinant Human EphB3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly34-Leu559. |
Product Background |
Although EphB3 expression is down-regulated in colorectal cancer (CRC) cells compared with normal intestinal epithelial cells.EphB3 is down-regulated in CRC compared to normal mucosa. Hypermethylation of CpG island is contributed to downregulation of EphB3 in CRC. EphB3 expression in tumor cells may be a useful prognostic indicator for patients with CRC. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
EphB3 |
Synonyms |
EK2; ETK2; HEK2; TYRO6; EPHB3; AW456895; Cek10; MDK5; Sek4; EC 2.7.10; EC 2.7.10.1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly34-Leu559 |
Molecular Weight |
The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human EphB3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EphB3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human EphB3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EphB3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. |
|
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Human EphB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human EphB3 is greater than 95% as determined by SEC-HPLC. |
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