• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human EphB3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EphB3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
  • Amino Acid Range: Gly34-Leu559
Catalog #EPH-HM1B3
Price
Regular price $297.00
Regular price Sale price $297.00
/
Size
Avantor VWR Fisher Scientific

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Human EphB3 Protein (EPH-HM1B3) Summary

Product Description

 Recombinant Human EphB3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly34-Leu559.

Product Background

 Although EphB3 expression is down-regulated in colorectal cancer (CRC) cells compared with normal intestinal epithelial cells.EphB3 is down-regulated in CRC compared to normal mucosa. Hypermethylation of CpG island is contributed to downregulation of EphB3 in CRC. EphB3 expression in tumor cells may be a useful prognostic indicator for patients with CRC.

Human EphB3 Protein (EPH-HM1B3) Specifications/Details

Protein
 EphB3
Synonyms
 EK2; ETK2; HEK2; TYRO6; EPHB3; AW456895; Cek10; MDK5; Sek4; EC 2.7.10; EC 2.7.10.1;EC2.7.10; EC2.7.10.1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly34-Leu559
Molecular Weight
 The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human EphB3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EphB3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human EphB3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EphB3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. Immobilized Human EphB3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EphB3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
Human EphB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human EphB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human EphB3 is greater than 95% as determined by SEC-HPLC. The purity of Human EphB3 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...