Name: Human EphB3 Protein, Ultra Low Endotoxin (EPH-HM1B3-UL)
Brand: KactusBio
SKU: EPH-HM1B3-UL-100UG
Price: 327.0 USD
Availability: InStock

Human EphB3 Protein, Ultra Low Endotoxin (EPH-HM1B3-UL)

Immobilized Human EphB3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EphB3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.

Human EphB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human EphB3 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human EphB3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EphB3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly34-Leu559

Catalog #EPH-HM1B3-UL

Size

100UG

500UG

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Product Description	Recombinant Human EphB3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly34-Leu559.
Product Background	Although EphB3 expression is down-regulated in colorectal cancer (CRC) cells compared with normal intestinal epithelial cells.EphB3 is down-regulated in CRC compared to normal mucosa. Hypermethylation of CpG island is contributed to downregulation of EphB3 in CRC. EphB3 expression in tumor cells may be a useful prognostic indicator for patients with CRC.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	EphB3
Synonyms	EK2; ETK2; HEK2; TYRO6; EPHB3; AW456895; Cek10; MDK5; Sek4; EC 2.7.10; EC 2.7.10.1
Accession	P54753
Species	Human
Biotinylated	no
Amino Acid Range	Gly34-Leu559
Molecular Weight	The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human EphB3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EphB3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human EphB3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EphB3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
	Human EphB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human EphB3 is greater than 95% as determined by SEC-HPLC.