HPLC chromatogram showing the purity profile of a recombinant protein sample with a single sharp peak at approximately 6.9 minutes, indicating high purity and molecular uniformity.
Human EphB4 Protein (EPB-HM104)
HPLC chromatogram showing the purity profile of a recombinant protein sample with a single sharp peak at approximately 6.9 minutes, indicating high purity and molecular uniformity.
Human EphB4 Protein (EPB-HM104)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Leu16-Ala539
Catalog #EPB-HM104
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Size
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Human EphB4 Protein (EPB-HM104) Summary

Product Description

 Recombinant Human EphB4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu16-Ala539.

Product Background

 The erythropoietin-producing hepatocellular carcinoma (Eph) receptors are the largest family of receptor tyrosine kinases (RTKs) that include two major subclasses, EphA and EphB. They form an important cell communication system with critical and diverse roles in a variety of biological processes during embryonic development. The emerging picture suggests that EphB4 is a valuable and attractive therapeutic target for upper aerodigestive malignancies.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human EphB4 Protein (EPB-HM104) Specifications/Details

Protein
 EphB4
Synonyms
 EPHB4; HTK; MYK1; TYRO11
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu16-Ala539
Molecular Weight
 The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human EphB4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human EphB4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human EphB4 is greater than 95% as determined by SEC-HPLC. The purity of Human EphB4 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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