Human Ephrin-A4/EFNA4 Protein (EPA-HM104)
Human Ephrin-A4/EFNA4 Protein (EPA-HM104)
Human Ephrin-A4/EFNA4 Protein (EPA-HM104)
Human Ephrin-A4/EFNA4 Protein (EPA-HM104)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.113 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu26-Gly171
Catalog #EPA-HM104
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Product Description
 Recombinant Human Ephrin-A4/EFNA4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu26-Gly171.
Product Background
 Ephrin A4 is one of the ephrin ligand molecules belonging to the tyrosine kinases receptor family. It was originally identified in a T-lymphoma cell line and seen to be expressed in human adult tissue as well as several tumor types. The cytoplasmic pattern of ephrin A4 could identify a subgroup of primary osteosarcoma patients with a high liability for progression, poor prognosis, and inferior response to chemotherapy.
Product Category
 Recombinant Protein
Target
 Ephrin-A4/EFNA4
Synonyms
 Ephrin-A4; LERK-4; EFNA4; EPLG4; EFL4
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu26-Gly171
Molecular Weight
 The protein has a predicted MW of 17.5 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 0.113 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human EphA7, His Tag immobilized on CM5 Chip can bind Human Ephrin-A4, His Tag with an affinity constant of 0.113 uM as determined in SPR assay (Biacore T200).
Human Ephrin-A4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Ephrin-A4 is greater than 95% as determined by SEC-HPLC.
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