Human Ephrin-A4/EFNA4 Protein (EPA-HM104)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.113 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Leu26-Gly171

Catalog: EPA-HM104

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Product Description	Recombinant Human Ephrin-A4/EFNA4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu26-Gly171.
Product Background	Ephrin A4 is one of the ephrin ligand molecules belonging to the tyrosine kinases receptor family. It was originally identified in a T-lymphoma cell line and seen to be expressed in human adult tissue as well as several tumor types. The cytoplasmic pattern of ephrin A4 could identify a subgroup of primary osteosarcoma patients with a high liability for progression, poor prognosis, and inferior response to chemotherapy.
Product Category	Recombinant Protein

Target	Ephrin-A4/EFNA4
Synonyms	Ephrin-A4; LERK-4; EFNA4; EPLG4; EFL4
Gene/Protein ID	P52798-1
Species	Human
Exact Sequence	Leu26-Gly171
Molecular Weight	The protein has a predicted MW of 17.5 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.113 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human EphA7, His Tag immobilized on CM5 Chip can bind Human Ephrin-A4, His Tag with an affinity constant of 0.113 uM as determined in SPR assay (Biacore T200).
	The purity of Human Ephrin-A4 is greater than 95% as determined by SEC-HPLC.
	Human Ephrin-A4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.