Human Ephrin-A4/EFNA4 Protein (EPA-HM104)
Human Ephrin-A4/EFNA4 Protein (EPA-HM104)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.113 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu26-Gly171
Catalog #EPA-HM104
Regular price $218.00
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Product Description

Recombinant Human Ephrin-A4/EFNA4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu26-Gly171.

Product Background

Ephrin A4 is one of the ephrin ligand molecules belonging to the tyrosine kinases receptor family. It was originally identified in a T-lymphoma cell line and seen to be expressed in human adult tissue as well as several tumor types. The cytoplasmic pattern of ephrin A4 could identify a subgroup of primary osteosarcoma patients with a high liability for progression, poor prognosis, and inferior response to chemotherapy.

Product Category

Recombinant Protein

Protein

Ephrin-A4/EFNA4

Synonyms

Ephrin-A4; Ephrin A4; LERK-4; EFNA4; EPLG4; EFL4

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Leu26-Gly171

Molecular Weight

The protein has a predicted MW of 17.5 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Bis-Tris PAGE result.

Activity

The affinity constant of 0.113 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human EphA7, His Tag immobilized on CM5 Chip can bind Human Ephrin-A4, His Tag with an affinity constant of 0.113 uM as determined in SPR assay (Biacore T200). Human EphA7, His Tag immobilized on CM5 Chip can bind Human Ephrin-A4, His Tag with an affinity constant of 0.113 uM as determined in SPR assay (Biacore T200).
Human Ephrin-A4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Ephrin-A4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Ephrin-A4 is greater than 95% as determined by SEC-HPLC. The purity of Human Ephrin-A4 is greater than 95% as determined by SEC-HPLC.

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