Human Ephrin-B2/EFNB2 Protein (ENB-HM201)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human EPHB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 82.97 pM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ile28-Ala229

Catalog: ENB-HM201

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Product Description Recombinant Human Ephrin-B2/EFNB2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ile28-Ala229.

Product Background

Ephrin-B2 controls platelet-derived growth factor receptor β (PDGFRβ) distribution in the VSMC plasma membrane, endocytosis, and signaling in a fashion that is highly distinct from its role in the endothelium. Ephrin-B2 is an important regulator of PDGFRβ endocytosis and thereby acts as a molecular switch controlling the downstream signaling activity of this receptor in mural cells.

Product Category

Recombinant Protein

Target

Ephrin-B2/EFNB2
Synonyms EPLG5; HTKL; LERK5; EFNB2

Gene/Protein ID

NP_004084.1

Species

Human

Exact Sequence

Ile28-Ala229
Molecular Weight The protein has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human EPHB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 82.97 pM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Ephrin-B2, hFc Tag captured on CM5 Chip via Protein A can bind Human EPHB2, His Tag with an affinity constant of 82.97 pM as determined in SPR assay (Biacore T200).
Immobilized Human EPHB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).
The purity of Human Ephrin B2 is greater than 95% as determined by SEC-HPLC.
Human Ephrin B2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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