• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human EPHB2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 1.3ng/ml determined by ELISA (QC Test). The affinity constant of 82.97 pM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ile28-Ala229
Catalog #ENB-HM201
Price
Regular price $14850
Regular price Sale price $14850
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Size
Avantor VWR Fisher Scientific

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Human Ephrin-B2/EFNB2 Protein (ENB-HM201) Summary

Product Description

 Recombinant Human Ephrin-B2/EFNB2 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ile28-Ala229.

Product Background

 Ephrin-B2 controls platelet-derived growth factor receptor β (PDGFRβ) distribution in the VSMC plasma membrane, endocytosis, and signaling in a fashion that is highly distinct from its role in the endothelium. Ephrin-B2 is an important regulator of PDGFRβ endocytosis and thereby acts as a molecular switch controlling the downstream signaling activity of this receptor in mural cells.

Human Ephrin-B2/EFNB2 Protein (ENB-HM201) Specifications/Details

Protein
 Ephrin-B2/EFNB2
Synonyms
 Ephrin B2; EPLG5; HTKL; LERK5; EFNB2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ile28-Ala229
Molecular Weight
 The protein has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human EPHB2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 1.3ng/ml determined by ELISA (QC Test). The affinity constant of 82.97 pM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Ephrin-B2, hFc Tag captured on CM5 Chip via Protein A can bind Human EPHB2, His Tag with an affinity constant of 82.97 pM as determined in SPR assay (Biacore T200). Human Ephrin-B2, hFc Tag captured on CM5 Chip via Protein A can bind Human EPHB2, His Tag with an affinity constant of 82.97 pM as determined in SPR assay (Biacore T200).
Immobilized Human EPHB2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 1.3ng/ml determined by ELISA (QC Test). Immobilized Human EPHB2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 1.3ng/ml determined by ELISA (QC Test).
Human Ephrin B2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Ephrin B2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Ephrin B2 is greater than 95% as determined by SEC-HPLC. The purity of Human Ephrin B2 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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