Name: Human Ephrin-B2/EFNB2 Protein (ENB-HM201)
Brand: KactusBio
SKU: ENB-HM201-100UG
Price: 135.0 USD
Availability: InStock

Human Ephrin-B2/EFNB2 Protein (ENB-HM201)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human EPHB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 82.97 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ile28-Ala229

Catalog #ENB-HM201

Size

100UG

500UG

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Product Description	Recombinant Human Ephrin-B2/EFNB2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ile28-Ala229.
Product Background	Ephrin-B2 controls platelet-derived growth factor receptor β (PDGFRβ) distribution in the VSMC plasma membrane, endocytosis, and signaling in a fashion that is highly distinct from its role in the endothelium. Ephrin-B2 is an important regulator of PDGFRβ endocytosis and thereby acts as a molecular switch controlling the downstream signaling activity of this receptor in mural cells.
Product Category	Recombinant Protein

Target	Ephrin-B2/EFNB2
Synonyms	EPLG5; HTKL; LERK5; EFNB2
Accession	NP_004084.1
Species	Human
Biotinylated	no
Amino Acid Range	Ile28-Ala229
Molecular Weight	The protein has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human EPHB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 82.97 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human Ephrin-B2, hFc Tag captured on CM5 Chip via Protein A can bind Human EPHB2, His Tag with an affinity constant of 82.97 pM as determined in SPR assay (Biacore T200).
	Immobilized Human EPHB2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).
	Human Ephrin B2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Ephrin B2 is greater than 95% as determined by SEC-HPLC.

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