Human EREG Protein (ERE-HM201) Summary
Product Description |
Recombinant Human EREG Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Val63-Leu108. |
Product Background |
Epidermal growth factor receptor (EGFR) regulates many crucial cellular programs, with seven different activating ligands shaping cell signaling in distinct ways.EGFR ligands epiregulin (EREG) and epigen (EPGN) stabilize different dimeric conformations of the EGFR extracellular region. As a consequence, EREG or EPGN induce less stable EGFR dimers than EGF-making them partial agonists of EGFR dimerization. Unexpectedly, this weakened dimerization elicits more sustained EGFR signaling than seen with EGF, provoking responses in breast cancer cells associated with differentiation rather than proliferation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human EREG Protein (ERE-HM201) Specifications/Details
Protein |
EREG |
Synonyms |
Proepiregulin; EPR; Epiregulin; EREG; ER |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val63-Leu108 |
Molecular Weight |
The protein has a predicted MW of 32.6 kDa. Due to glycosylation, the protein migrates to 37-42 kDa based on Bis-Tris PAGE result. |
Product Tag |
N-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human EREG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human EREG is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from Antibody Drug Discovery Proteins
Recently viewed
