Human Fas Ligand/TNFSF6 Protein (FAS-HM10L)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human Fas Ligand, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 75.0ng/ml determined by ELISA (QC Test). The affinity constant of 19.45 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Pro134-Leu281
Catalog #FAS-HM10L
/
Size

Order now, ships by:

Product Description
 Recombinant Human Fas Ligand/TNFSF6 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Pro134-Leu281.
Product Background
 Fas ligand (FasL, CD95L) is a 40-kDa type II transmembrane protein that binds to Fas (CD95) receptors and promotes programmed cell death. Fas receptors are expressed at higher levels in many tumors than in normal cells.Somewhat paradoxically, elimination of Fas or FasL from tumors also leads to death induced by CD95 receptor/ligand elimination (DICE).
Product Category
 Recombinant Protein
Target
 Fas Ligand/TNFSF6
Synonyms
 APTL; CD95-L; Fas ligand; APT1LG1; TNFSF6; FASLG; ALPS1B; CD178; FASL; TNLG1A
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Pro134-Leu281
Molecular Weight
 The protein has a predicted MW of 18 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Fas Ligand, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 75.0ng/ml determined by ELISA (QC Test). The affinity constant of 19.45 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 N-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Fas, His Tag immobilized on CM5 Chip can bind Human Fas Ligand, His Tag with an affinity constant of 19.45 nM as determined in SPR assay (Biacore T200).
Immobilized Human Fas Ligand, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 75.0ng/ml determined by ELISA (QC Test).
Human Fas Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed