Human Fas Ligand/TNFSF6 Protein (FAS-HM10L)
Catalog: FAS-HM10L
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Product Description | Recombinant Human Fas Ligand/TNFSF6 Protein is expressed from Expi293 with His tag at the N-terminal.It contains Pro134-Leu281. |
Product Category | Recombinant Protein |
Target | Fas Ligand/TNFSF6 |
Synonyms | APTL; CD95-L; Fas ligand; APT1LG1; TNFSF6; FASLG; ALPS1B; CD178; FASL; TNLG1A |
Gene/Protein ID | AAH17502.1 |
Species | Human |
Exact Sequence | Pro134-Leu281 |
Molecular Weight | The protein has a predicted MW of 18 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human Fas Ligand, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 0.19μg/ml determined by ELISA (QC Test). The affinity constant of 19.45 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | N-His |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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