Human Fas Ligand/TNFSF6 Protein (FAS-HM10L)

  • Express System: Expi293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human Fas Ligand, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. The affinity constant of 19.45 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Pro134-Leu281

Catalog: FAS-HM10L

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Product Description Recombinant Human Fas Ligand/TNFSF6 Protein is expressed from Expi293 with His tag at the N-terminal.It contains Pro134-Leu281.
Product Category Recombinant Protein
Target Fas Ligand/TNFSF6
Synonyms APTL; CD95-L; Fas ligand; APT1LG1; TNFSF6; FASLG; ALPS1B; CD178; FASL; TNLG1A
Gene/Protein ID AAH17502.1
Species Human
Exact Sequence Pro134-Leu281
Molecular Weight The protein has a predicted MW of 18 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Fas Ligand, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. The affinity constant of 19.45 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Fas, His Tag immobilized on CM5 Chip can bind Human Fas Ligand, His Tag with an affinity constant of 19.45 nM as determined in SPR assay (Biacore T200).
Immobilized Human Fas Ligand, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA.
Human Fas Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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