Human Fas Ligand/TNFSF6 Protein (FAS-HM10L)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human Fas Ligand, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 75.0ng/ml determined by ELISA (QC Test). The affinity constant of 19.45 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Pro134-Leu281

Catalog: FAS-HM10L

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Product Description Recombinant Human Fas Ligand/TNFSF6 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Pro134-Leu281.

Product Background

Fas ligand (FasL, CD95L) is a 40-kDa type II transmembrane protein that binds to Fas (CD95) receptors and promotes programmed cell death. Fas receptors are expressed at higher levels in many tumors than in normal cells.Somewhat paradoxically, elimination of Fas or FasL from tumors also leads to death induced by CD95 receptor/ligand elimination (DICE).

Product Category

Recombinant Protein

Target

Fas Ligand/TNFSF6
Synonyms APTL; CD95-L; Fas ligand; APT1LG1; TNFSF6; FASLG; ALPS1B; CD178; FASL; TNLG1A
Accession

Species

Human

Amino Acid Range

Pro134-Leu281
Molecular Weight The protein has a predicted MW of 18 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Fas Ligand, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 75.0ng/ml determined by ELISA (QC Test). The affinity constant of 19.45 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Fas, His Tag immobilized on CM5 Chip can bind Human Fas Ligand, His Tag with an affinity constant of 19.45 nM as determined in SPR assay (Biacore T200).
Immobilized Human Fas Ligand, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 75.0ng/ml determined by ELISA (QC Test).
Human Fas Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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