Product Description |
Recombinant Human Fas/TNFRSF6/CD95 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln26-Asn173. |
Product Background |
CD95 (also known as Fas) is a member of the tumor necrosis factor receptor (TNFR) superfamily. Its cognate ligand, CD95L, is implicated in immune homeostasis and immune surveillance. Mutations in this receptor are associated with a loss of apoptotic signaling and have been detected in an autoimmune disorder called autoimmune lymphoproliferative syndrome (ALPS) type Ia, which shares some clinical features with systemic lupus erythematosus (SLE). |
Product Category |
Recombinant Protein |
Target |
Fas/TNFRSF6/CD95 |
Synonyms |
FASLG receptor; CD95; APT1; FAS1; TNFRSF6; FAS; ALPS1A; APO1; FASTM; FasR |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln26-Asn173 |
Molecular Weight |
The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Fas Ligand, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 75.0ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human Fas Ligand, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 75.0ng/ml determined by ELISA. |
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Human Fas on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Fas is greater than 95% as determined by SEC-HPLC. |
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