Human Fas/TNFRSF6/CD95 Protein (FAS-HM201)
Catalog: FAS-HM201
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| Product Description | Recombinant Human Fas/TNFRSF6/CD95 Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Gln26-Asn173. |
| Product Category | Recombinant Protein |
| Target | Fas/TNFRSF6/CD95 |
| Synonyms | FASLG receptor; CD95; APT1; FAS1; TNFRSF6; FAS; ALPS1A; APO1; FASTM; FasR |
| Gene/Protein ID | P25445-1 |
| Species | Human |
| Exact Sequence | Gln26-Asn173 |
| Molecular Weight | The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human Fas Ligand, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human Fas Ligand, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. |
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The purity of Human Fas is greater than 95% as determined by SEC-HPLC. |
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Human Fas on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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