Human Fc epsilon RI alpha/FCER1a Protein (FER-HM201)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 10ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val26-Gln205

Catalog: FER-HM201

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Product Description Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Val26-Gln205.
Product Category Recombinant Protein
Target Fc epsilon RI alpha/FCER1a
Synonyms Fc-epsilon RI-alpha; FcERI; FCER1A; FCE1A
Gene/Protein ID P12319-1
Species Human
Exact Sequence Val26-Gln205
Molecular Weight The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 10ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 10ng/ml determined by ELISA.
The purity of Human Fc epsilon RI alpha is greater than 95% as determined by SEC-HPLC.
Human Fc epsilon RI alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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