Name: Human Fc epsilon RI alpha/FCER1a Protein (FER-HM201)
Brand: KactusBio
SKU: FER-HM201-100UG
Price: 223.3 USD
Availability: InStock

Human Fc epsilon RI alpha/FCER1a Protein (FER-HM201)

Immobilized Human Fc epsilon RI alpha, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 28.7ng/ml determined by ELISA (QC Test).

Immobilized Human Fc epsilon RI alpha, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IgE, His Tag with the EC50 of 3.3ng/ml determined by ELISA.

Human Fc epsilon RI alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Fc epsilon RI alpha is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human Fc epsilon RI alpha, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 28.7ng/ml determined by ELISA (QC Test).
Amino Acid Range: Val26-Gln205

Catalog #FER-HM201

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human Fc epsilon RI alpha/FCER1a Protein (FER-HM201) Summary

Product Description	Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val26-Gln205.
Product Background	Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels.
Product Category	Recombinant Protein / Antibody discovery / Fc receptors

Human Fc epsilon RI alpha/FCER1a Protein (FER-HM201) Specifications/Details

Protein	Fc epsilon RI alpha/FCER1a
Synonyms	Fc-epsilon RI-alpha; FcERI; FCER1A; FCE1A
Accession	P12319-1
Species	Human
Biotinylated	no
Amino Acid Range	Val26-Gln205
Molecular Weight	The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Fc epsilon RI alpha, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 28.7ng/ml determined by ELISA (QC Test).
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human Fc epsilon RI alpha, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 28.7ng/ml determined by ELISA (QC Test).
	Immobilized Human Fc epsilon RI alpha, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IgE, His Tag with the EC50 of 3.3ng/ml determined by ELISA.
	Human Fc epsilon RI alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Fc epsilon RI alpha is greater than 90% as determined by SEC-HPLC.