Product Description |
Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val26-Gln205. |
Product Background |
Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels. |
Product Category |
Recombinant Protein |
Target |
Fc epsilon RI alpha/FCER1a |
Synonyms |
Fc-epsilon RI-alpha; FcERI; FCER1A; FCE1A |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val26-Gln205 |
Molecular Weight |
The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 14.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 14.5ng/ml determined by ELISA. |
|
|
Human Fc epsilon RI alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Fc epsilon RI alpha is greater than 95% as determined by SEC-HPLC. |
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