Human Fc epsilon RI alpha/FCER1a Protein (FER-HM201)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 14.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val26-Gln205

Catalog: FER-HM201

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Product Description Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val26-Gln205.

Product Background

Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels.

Product Category

Recombinant Protein

Target

Fc epsilon RI alpha/FCER1a
Synonyms Fc-epsilon RI-alpha; FcERI; FCER1A; FCE1A

Gene/Protein ID

P12319-1

Species

Human

Exact Sequence

Val26-Gln205
Molecular Weight The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 14.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 14.5ng/ml determined by ELISA.
The purity of Human Fc epsilon RI alpha is greater than 95% as determined by SEC-HPLC.
Human Fc epsilon RI alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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