Human Fc gamma RI/CD64 Protein (FRI-HM464)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 1.94 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln16-Pro288

Catalog: FRI-HM464

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Product Description Recombinant Human Fc gamma RI/CD64 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gln16-Pro288.
Product Category Recombinant Protein
Target Fc gamma RI/CD64
Synonyms CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI
Gene/Protein ID P12314-1
Species Human
Exact Sequence Gln16-Pro288
Molecular Weight The protein has a predicted MW of 33.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 1.94 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Fc gamma RI, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 1.94 nM as determined in SPR assay (Biacore T200).
The purity of Human Fc gamma RI is greater than 95% as determined by SEC-HPLC.
Human Fc gamma RI on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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