Human Fc gamma RI/CD64 Protein (FRI-HM464)
Catalog: FRI-HM464
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| Product Description | Recombinant Human Fc gamma RI/CD64 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gln16-Pro288. |
| Product Category | Recombinant Protein |
| Target | Fc gamma RI/CD64 |
| Synonyms | CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI |
| Gene/Protein ID | P12314-1 |
| Species | Human |
| Exact Sequence | Gln16-Pro288 |
| Molecular Weight | The protein has a predicted MW of 33.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 1.94 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human Fc gamma RI, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 1.94 nM as determined in SPR assay (Biacore T200). |
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The purity of Human Fc gamma RI is greater than 95% as determined by SEC-HPLC. |
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Human Fc gamma RI on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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