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Human Fc gamma RIIA/CD32a (H167) Protein (CDA-HM132) Summary
Product Description |
Recombinant Human Fc gamma RIIA/CD32a (H167) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln34-Ile218(H167). |
Product Background |
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. |
Product Category |
Recombinant Protein / Antibody discovery / Fc receptors |
Human Fc gamma RIIA/CD32a (H167) Protein (CDA-HM132) Specifications/Details
Protein |
Fc gamma RIIA/CD32a |
Synonyms |
FcgammaRIIA; Fc gammaRIIA; Fcgamma RIIA; Fc gamma RIIA; FcγRIIA; Fc γRIIA; Fcγ RIIA; Fc γ RIIA; FcgRIIA; Fc gRIIA; Fcg RIIA; Fc g RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FCRIIA; fcRII-a; FCG2; CD32A |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln34-Ile218(H167) |
Molecular Weight |
The protein has a predicted MW of 22.12 kDa. Due to glycosylation, the protein migrates to 32-40 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 5.72 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human Fc gamma RIIA (H167), His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 5.72 uM as determined in SPR assay (Biacore S200). |
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Human Fc gamma RIIA (H167) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Human Fc gamma RIIA (H167), His Tag on Anti-His-Biosensor can bind Rituximab with an affinity constant of 0.36 µM as determined in BLI assay. |
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The purity of Human Fc gamma RIIA (H167) is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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