Human Fc gamma RIIA/CD32a (R167) Protein (FCR-HM224)

Human Fc gamma RIIA (R167), hFc Tag immobilized on CM5 Chip can bind Rituximab, hFc Tag with an affinity constant of 3.86 uM as determined in SPR assay (Biacore T200).

Human Fc gamma RIIA (R167) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Fc gamma RIIA (R167) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 3.86 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gln34-Ile218(R167)

Catalog #FCR-HM224

Price

$315⁰⁰

Size

Order by 4pm ET for delivery on:

Human Fc gamma RIIA/CD32a (R167) Protein (FCR-HM224) Summary

Product Description

Recombinant Human Fc gamma RIIA/CD32a (R167) Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Gln34-Ile218(R167).

Product Background

The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.

Human Fc gamma RIIA/CD32a (R167) Protein (FCR-HM224) Specifications/Details

Protein	Fc gamma RIIA/CD32a
Synonyms	Fc gamma RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; FCG2; CD32A; FcγRIIA; Fc γRIIA; Fcγ RIIA; Fc γ RIIA;Fc gRIIA; Fcg RIIA; Fc g RIIA
Accession	P12318-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln34-Ile218(R167)
Molecular Weight	The protein has a predicted MW of 46.47 kDa. Due to glycosylation, the protein migrates to 53-65 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 3.86 uM as determined in SPR assay (Biacore T200).
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human Fc gamma RIIA (R167), hFc Tag immobilized on CM5 Chip can bind Rituximab, hFc Tag with an affinity constant of 3.86 uM as determined in SPR assay (Biacore T200).
	Human Fc gamma RIIA (R167) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Fc gamma RIIA (R167) is greater than 95% as determined by SEC-HPLC.