Human Fc gamma RIIB/CD32b Protein (CDB-HM401)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 9.4_M as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala46-Pro217

Catalog: CDB-HM401

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Product Description Recombinant Human Fc gamma RIIB/CD32b Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ala46-Pro217.
Product Category Recombinant Protein
Target Fc gamma RIIB/CD32b
Synonyms Fc gamma Receptor IIB/C; CD32; CD32b; CDw32; FCGR2B/C; CD32b/c; FcRII-b/c; FCG2; IGFR2; CD32A; Fc gamma RII; Fc gamma RIIB; FCG2 ; FcGR; FCGR2; FCGR21; FCGR2A
Gene/Protein ID P31994-1
Species Human
Exact Sequence Ala46-Pro217
Molecular Weight The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 9.4_M as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Fc gamma RIIB, His Tag captured on CM5 Chip via anti-His antibody can bind Rituximab, hFc Tag with an affinity constant of 9.4_M as determined in SPR assay (Biacore T200).
The purity of Human Fc gamma RIIB is greater than 95% as determined by SEC-HPLC.
Human Fc gamma RIIB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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