Human Fc gamma RIIB/CD32b Protein (CDB-HM401)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 3.46 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala46-Pro217

Catalog: CDB-HM401

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Product Description Recombinant Human Fc gamma RIIB/CD32b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala46-Pro217.

Product Background

The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.

Product Category

Recombinant Protein

Target

Fc gamma RIIB/CD32b
Synonyms Fc gamma Receptor IIB/C; CD32; CD32b; CDw32; FCGR2B/C; CD32b/c; FcRII-b/c; FCG2; IGFR2; CD32A; Fc gamma RII; Fc gamma RIIB; FCG2 ; FcGR; FCGR2; FCGR21; FCGR2A
Accession

Species

Human

Amino Acid Range

Ala46-Pro217
Molecular Weight The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 3.46 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIB, His Tag with an affinity constant of 3.46 uM as determined in SPR assay (Biacore T200).
The purity of Human Fc gamma RIIB is greater than 95% as determined by SEC-HPLC.
Human Fc gamma RIIB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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