Human Fc gamma RIIB/CD32b Protein (CDB-HM401)
Catalog: CDB-HM401
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| Product Description | Recombinant Human Fc gamma RIIB/CD32b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala46-Pro217. |
| Product Category | Recombinant Protein |
| Target | Fc gamma RIIB/CD32b |
| Synonyms | Fc gamma Receptor IIB/C; CD32; CD32b; CDw32; FCGR2B/C; CD32b/c; FcRII-b/c; FCG2; IGFR2; CD32A; Fc gamma RII; Fc gamma RIIB; FCG2 ; FcGR; FCGR2; FCGR21; FCGR2A |
| Gene/Protein ID | P31994-1 |
| Species | Human |
| Exact Sequence | Ala46-Pro217 |
| Molecular Weight | The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 3.46 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIB, His Tag with an affinity constant of 3.46 uM as determined in SPR assay (Biacore T200). |
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The purity of Human Fc gamma RIIB is greater than 95% as determined by SEC-HPLC. |
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Human Fc gamma RIIB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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