• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Fc gamma RIIIA (F176), His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA.
  • Amino Acid Range: Gly17-Gln208(F176)
Catalog #CDA-HM116
Price
Regular price $240.00
Regular price Sale price $240.00
/
Size
Avantor VWR Fisher Scientific

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Human Fc gamma RIIIA/CD16a (F176) Protein (CDA-HM116) Summary

Product Description

 Recombinant Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly17-Gln208(F176).

Product Background

 Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Product Category

 Recombinant Protein / Antibody discovery / Fc receptors

Human Fc gamma RIIIA/CD16a (F176) Protein (CDA-HM116) Specifications/Details

Protein
 Fc gamma RIIIA/CD16a
Synonyms
 Fc gamma RIIIA; Fc gamma RIIIA (V176); FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20; FcγRIIIA; Fc γRIIIA; Fcγ RIIIA; Fc γ RIIIA
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly17-Gln208(F176)
Molecular Weight
 The protein has a predicted MW of 23.44 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Fc gamma RIIIA (F176), His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Fc gamma RIIIA (F176), His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. Immobilized Human Fc gamma RIIIA (F176), His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA.
Human Fc gamma RIIIA (F176) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Fc gamma RIIIA (F176) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC. The purity of Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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