Human Fc gamma RIIIA/CD16a (F176) Protein (CDA-HM416)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Fc gamma RIIIA (F176) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.92uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly17-Gln208(F176)

Catalog: CDA-HM416

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Product Description Recombinant Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus..It contains Gly17-Gln208(F176).

Product Background

Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Product Category

Recombinant Protein

Target

Fc gamma RIIIA/CD16a (F176)
Synonyms CD16FCRIIIA; Fc fragment of IgG; Fc gamma RIIIA; Fc-gamma RIII-alpha; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20
Accession

Species

Human

Amino Acid Range

Gly17-Gln208(F176)
Molecular Weight The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Fc gamma RIIIA (F176) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.92uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via anti-His antibody can bind Rituximab, hFc Tag with an affinity constant of 0.92uM as determined in SPR assay (Biacore T200).
Immobilized Human Fc gamma RIIIA (F176) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA (QC Test).
The purity of Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC.
Human Fc gamma RIIIA (F176) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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