Human Fc gamma RIIIA/CD16a (F176) Protein (CDA-HM416)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Fc gamma RIIIA (F176) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.92uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly17-Gln208(F176)
Catalog #CDA-HM416
Regular price $264.00
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Product Description

Recombinant Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus..It contains Gly17-Gln208(F176).

Product Background

Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Product Category

Recombinant Protein / Antibody discovery / Fc receptors

Protein

Fc gamma RIIIA/CD16a

Synonyms

Fc gamma RIIIA; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20; FcγRIIIA; Fc γRIIIA; Fcγ RIIIA; Fc γ RIIIA

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gly17-Gln208(F176)

Molecular Weight

The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human Fc gamma RIIIA (F176) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.92uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via anti-His antibody can bind Rituximab, hFc Tag with an affinity constant of 0.92uM as determined in SPR assay (Biacore T200). Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via anti-His antibody can bind Rituximab, hFc Tag with an affinity constant of 0.92uM as determined in SPR assay (Biacore T200).
Immobilized Human Fc gamma RIIIA (F176) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA (QC Test). Immobilized Human Fc gamma RIIIA (F176) , His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA (QC Test).
Human Fc gamma RIIIA (F176) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human Fc gamma RIIIA (F176) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC. The purity of Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC.

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