Human Fc gamma RIIIA/CD16a (V176) Domain 2 Protein (FCR-HM31D)
Human Fc gamma RIIIA/CD16a (V176) Domain 2 Protein (FCR-HM31D)
  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly107-Thr189
Catalog #FCR-HM31D
Regular price $346.50
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Human Fc gamma RIIIA/CD16a (V176) Domain 2 Protein (FCR-HM31D) Summary

Product Description

Recombinant Human Fc gamma RIIIA/CD16a (V176) Domain 2 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminus.It contains Gly107-Thr189.

Product Background

Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Product Category

Recombinant Protein / Antibody discovery / Fc receptors

Human Fc gamma RIIIA/CD16a (V176) Domain 2 Protein (FCR-HM31D) Specifications/Details

Protein
Fc gamma RIIIA/CD16a
Synonyms
Fc gamma RIIIA; Fc gamma RIIIA (V176); FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20; FcγRIIIA; Fc γRIIIA; Fcγ RIIIA; Fc γ RIIIA
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gly107-Thr189
Molecular Weight
The protein has a predicted MW of 35.29 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Product Tag
C-mFc
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Fc gamma RIIIA (V176) Domain 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Fc gamma RIIIA (V176) Domain 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Fc gamma RIIIA (V176) Domain 2 is greater than 95% as determined by SEC-HPLC. The purity of Human Fc gamma RIIIA (V176) Domain 2 is greater than 95% as determined by SEC-HPLC.
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