Human Fc gamma RIIIB/CD16b (NA1) Protein (FCR-HM11B) Summary
Product Description |
Recombinant Human Fc gamma RIIIB/CD16b (NA1) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly17-Ser200(NA1). |
Product Background |
Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils. |
Product Category |
Recombinant Protein / Antibody discovery / Fc receptors |
Human Fc gamma RIIIB/CD16b (NA1) Protein (FCR-HM11B) Specifications/Details
Protein |
Fc gamma RIIIB/CD16b |
Synonyms |
CD16; Fc gamma RIIIB; FCG3; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10; FcγRIIIB; Fc γRIIIB; Fcγ RIIIB; Fc γ RIIIB |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly17-Ser200(NA1) |
Molecular Weight |
The protein has a predicted MW of 22.52 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 8.55 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human Fc gamma RIIIB (NA1), His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 8.55 uM as determined in SPR assay (Biacore T200). |
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Human Fc gamma RIIIB (NA1) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Human Fc gamma RIIIB (NA1), His Tag on Anti-His-Biosensor can bind Rituximab with an affinity constant of 2.27 µM as determined in BLI assay (Gator® Prime). |
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The purity of Human Fc gamma RIIIB (NA1) is greater than 95% as determined by SEC-HPLC. |
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