Human Fc gamma RIIIB/CD16b (NA2) Protein (FCR-HM42B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 2.33 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly17-Ser200(NA2)

Catalog: FCR-HM42B

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Product Description Recombinant Human Fc gamma RIIIB/CD16b (NA2) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly17-Ser200(NA2). The CD16b NA1 differ with the CD16b NA2 in AA36, 65, 82, and 106.The CD16b NA1 form carries R36, N65, D82, and V106, while the CD16b NA2 form carries S36,S65, N82, and I106.

Product Background

Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.

Product Category

Recombinant Protein

Target

Fc gamma RIIIB/CD16b (NA2)
Synonyms CD16; Fc gamma RIIIB; FCG3; Fc-gamma receptor IIIb (CD 16); Fc-gamma RIIIb; Fc-gamma RIII-beta; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; IgG Fc receptor III-1; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10
Accession

Species

Human

Amino Acid Range

Gly17-Ser200(NA2)
Molecular Weight The protein has a predicted MW of 23.7 kDa. Due to glycosylation, the protein migrates to 47-53 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 2.33 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Fc gamma RIIIB (NA2), His Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 2.33 uM as determined in SPR assay (Biacore T200).
The purity of Human Fc gamma RIIIB (NA2) is greater than 95% as determined by SEC-HPLC.
Human Fc gamma RIIIB (NA2) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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