Human Fc gamma RIIIB/CD16b (NA2) Protein (FCR-HM42B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 1.3μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly17-Ser200

Catalog: FCR-HM42B

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Product Description Recombinant Human Fc gamma RIIIB/CD16b (NA2) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gly17-Ser200. The CD16b NA1 differ with the CD16b NA2 in AA36, 65, 82, and 106.The CD16b NA1 form carries R36, N65, D82, and V106, while the CD16b NA2 form carries S36,S65, N82, and I106.
Product Category Recombinant Protein
Target Fc gamma RIIIB/CD16b (NA2)
Synonyms CD16; Fc gamma RIIIB; FCG3; Fc-gamma receptor IIIb (CD 16); Fc-gamma RIIIb; Fc-gamma RIII-beta; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; IgG Fc receptor III-1; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10
Gene/Protein ID O75015-1
Species Human
Exact Sequence Gly17-Ser200
Molecular Weight The protein has a predicted MW of 23.7 kDa. Due to glycosylation, the protein migrates to 47-53 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 1.3μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Fc gamma RIIIB, His Tag captured on CM5 Chip via anti-His antibody can bind Rituximab, hFc Tag with an affinity constant of 1.3μM as determined in SPR assay (Biacore T200).
The purity of Human Fc gamma RIIIB/CD16b is greater than 95% as determined by SEC-HPLC.
Human Fc gamma RIIIB/CD16b on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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