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Human FcRL2/IRTA4 Protein (FCR-HM102) Summary
Product Description |
Recombinant Human FcRL2/IRTA4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu20-Val401. |
Product Background |
Fc receptor-like 2 (FCRL2) is highly expressed on B-cell chronic lymphocytic leukemia (B-CLL) cells and could possibly influence disease pathogenesis. FCRL2 mRNA expression was found to be expressed at considerably higher levels in peripheral blood mononuclear cells (PBMC) of B-CLL patients compared to controls (range 1.35- to 210-fold upregulation; P < 0.0001) and cells of other hematological diseases. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human FcRL2/IRTA4 Protein (FCR-HM102) Specifications/Details
Protein |
FcRL2/IRTA4 |
Synonyms |
FcR-like protein 2; FcRL2; FCRH2; IFGP4; IRTA4; FCRL2; CD307b; FCRH2SPAP1B; IRTA4SPAP1C; SPAP1; SPAP1A |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu20-Val401 |
Molecular Weight |
The protein has a predicted MW of 42.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human FcRL2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FcRL2 Antibody, hFc Tag with the EC50 of 56.8ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human FcRL2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FcRL2 Antibody, hFc Tag with the EC50 of 56.8ng/ml determined by ELISA (QC Test). |
|
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Human FcRL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human FcRL2 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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