- Express System: HEK293
- Product Tag: C-hFc (IgG1)
- Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
- Activity: Immobilized Human FCRL2, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-FCRL2 Antibody, hFc Tag with the EC50 of 89.4ng/ml determined by ELISA.
- Amino Acid Range: Leu20-Val401
Human FcRL2/IRTA4 Protein (FCR-HM202) Summary
Product Description |
Recombinant Human FcRL2/IRTA4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Leu20-Val401. |
Product Background |
Fc receptor-like 2 (FCRL2) is highly expressed on B-cell chronic lymphocytic leukemia (B-CLL) cells and could possibly influence disease pathogenesis. FCRL2 mRNA expression was found to be expressed at considerably higher levels in peripheral blood mononuclear cells (PBMC) of B-CLL patients compared to controls (range 1.35- to 210-fold upregulation; P < 0.0001) and cells of other hematological diseases. |
Human FcRL2/IRTA4 Protein (FCR-HM202) Specifications/Details
Protein |
FcRL2/IRTA4 |
Synonyms |
FcR-like protein 2; FcRL2; FCRH2; IFGP4; IRTA4; FCRL2; CD307b; FCRH2SPAP1B; IRTA4SPAP1C; SPAP1; SPAP1A |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu20-Val401 |
Molecular Weight |
The protein has a predicted MW of 67.58 kDa. Due to glycosylation, the protein migrates to 85-105 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human FCRL2, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-FCRL2 Antibody, hFc Tag with the EC50 of 89.4ng/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
Other Available Proteins: