Name: Human FcRn Protein (FRN-HM101)
Brand: KactusBio
SKU: FRN-HM101-100UG
Price: 355.3 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human FcRn, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 47.5 ng/ml determined by ELISA (QC Test). The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)

Catalog #FRN-HM101

Size

100UG

500UG

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Human FcRn Protein (FRN-HM101) Summary

Product Description	Recombinant Human FcRn Protein is expressed from HEK293 with His tag at the C-Terminus of FCGRT.It contains Ala24-Ser297(FCGRT) and Ile21-Met119(B2M).
Product Background	The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.
Product Category	Recombinant Protein / Antibody discovery / Fc receptors

Human FcRn Protein (FRN-HM101) Specifications/Details

Protein	FcRn
Synonyms	FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
Accession	P55899-1(FCGRT)&P61769-1(B2M)
Species	Human
Biotinylated	no
Amino Acid Range	Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)
Molecular Weight	The protein has a predicted MW of 31.5 kDa (FCGRT)&11.7 kDa (B2M). Due to glycosylation, the protein migrates to 35-40 kDa (FCGRT)&13-14 kDa (B2M) based on Bis-Tris PAGE result.
Activity	Immobilized Human FcRn, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 47.5 ng/ml determined by ELISA (QC Test). The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.28 uM as determined in SPR assay (Biacore T200).
	Immobilized Human FcRn, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 47.5 ng/ml determined by ELISA (QC Test).
	Human FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human FcRn, His Tag on Anti-His-Biosensor can bind Human IgG4 Fc, No Tag (Cat. IGG-HM004) with an affinity constant of 30.30 nM as determined in BLI assay.
	The purity of Human FcRn is greater than 95% as determined by SEC-HPLC.

Other Available Proteins:

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