Human FcRn Protein (FRN-HM101)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FcRn, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA. The affinity constant of 0.40 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)

Catalog: FRN-HM101

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Product Description Recombinant Human FcRn Protein is expressed from Expi293 with His tag at the C-terminal.It contains Ala24-Ser297(FCGRT) & Ile21-Met119(B2M).
Product Category Recombinant Protein
Target FcRn
Synonyms FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
Gene/Protein ID P55899-1(FCGRT)&P61769-1(B2M)
Species Human
Exact Sequence Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)
Molecular Weight The protein has a predicted MW of 31.5 kDa (FCGRT) & 11.7 kDa (B2M). Due to glycosylation, the protein migrates to 35-40 kDa (FCGRT) & 13-14 kDa (B2M) based on Tris-Bis PAGE result.
Activity Immobilized Human FcRn, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA. The affinity constant of 0.40 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.40 μM as determined in SPR assay (Biacore T200).
Immobilized Human FcRn, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA.
The purity of Human FcRn is greater than 95% as determined by SEC-HPLC.
Human FcRn on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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