Human FcRn Protein (FRN-HM101)
Catalog: FRN-HM101
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Product Description | Recombinant Human FcRn Protein is expressed from Expi293 with His tag at the C-terminal.It contains Ala24-Ser297(FCGRT) & Ile21-Met119(B2M). |
Product Category | Recombinant Protein |
Target | FcRn |
Synonyms | FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M |
Gene/Protein ID | P55899-1(FCGRT)&P61769-1(B2M) |
Species | Human |
Exact Sequence | Ala24-Ser297(FCGRT) & Ile21-Met119(B2M) |
Molecular Weight | The protein has a predicted MW of 31.5 kDa (FCGRT) & 11.7 kDa (B2M). Due to glycosylation, the protein migrates to 35-40 kDa (FCGRT) & 13-14 kDa (B2M) based on Tris-Bis PAGE result. |
Activity | Immobilized Human FcRn, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 11.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.40 μM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | C-His |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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