Human FcRn Protein (FRN-HM101)
Human FcRn Protein (FRN-HM101)
Human FcRn Protein (FRN-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human FcRn, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 5.4 ng/ml determined by ELISA (QC Test). The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)
Catalog #FRN-HM101
Regular price $323.00
/
Size

Order now, ships by:

Product Description

Recombinant Human FcRn Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala24-Ser297(FCGRT) & Ile21-Met119(B2M).

Product Background

The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.

Product Category

Recombinant Protein

Protein

FcRn

Synonyms

FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)

Molecular Weight

The protein has a predicted MW of 31.5 kDa (FCGRT)&11.7 kDa (B2M). Due to glycosylation, the protein migrates to 35-40 kDa (FCGRT)&13-14 kDa (B2M) based on Bis-Tris PAGE result.

Activity

Immobilized Human FcRn, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 5.4 ng/ml determined by ELISA (QC Test). The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.28 uM as determined in SPR assay (Biacore T200).
Immobilized Human FcRn, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 5.4 ng/ml determined by ELISA (QC Test). Immobilized Human FcRn, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 5.4 ng/ml determined by ELISA (QC Test).
Human FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human FcRn is greater than 95% as determined by SEC-HPLC. The purity of Human FcRn is greater than 95% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed