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Human FcRn Protein, Ultra Low Endotoxin (FRN-HM101-UL) Summary
Product Description |
Recombinant Human FcRn Protein is expressed from HEK293 with His tag at the C-Terminus of FCGRT.It contains Ala24-Ser297(FCGRT) and Ile21-Met119(B2M). |
Product Background |
The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer. |
Product Category |
Recombinant Protein / Antibody discovery / Fc receptors |
Human FcRn Protein, Ultra Low Endotoxin (FRN-HM101-UL) Specifications/Details
Protein |
FcRn |
Synonyms |
FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala24-Ser297(FCGRT) & Ile21-Met119(B2M) |
Molecular Weight |
The protein has a predicted MW of 31.5 kDa (FCGRT)&11.7 kDa (B2M). Due to glycosylation, the protein migrates to 35-40 kDa (FCGRT)&13-14 kDa (B2M) based on Bis-Tris PAGE result. |
Activity |
Immobilized Human FcRn, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 47.5 ng/ml determined by ELISA (QC Test). The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human FcRn, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 47.5 ng/ml determined by ELISA (QC Test). |
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Human FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Human FcRn, His Tag on Anti-His-Biosensor can bind Human IgG4 Fc, No Tag (Cat. IGG-HM004) with an affinity constant of 30.30 nM as determined in BLI assay. |
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The purity of Human FcRn is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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