Product Description |
Recombinant Human FGF-7/KGF Protein is expressed from E.coli with His tag at the N-Terminus.It contains Cys32-Thr194. |
Product Background |
The expression patterns of mRNAs encoding Fibroblast Growth Factor-7 (FGF-7) and its high affinity receptor suggested that FGF-7 signaling may play a role in regulating ureteric bud growth.t Results of these studies demonstrate that the developing ureteric bud and mature collecting system of FGF-7-null kidneys is markedly smaller than wild type. FGF-7 levels modulate the extent of ureteric bud growth during development and the number of nephrons that eventually form in the kidney. |
Product Category |
Recombinant Protein |
Target |
FGF-7/KGF |
Synonyms |
FGF-7; HBGF-7; KGF |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Cys32-Thr194 |
Molecular Weight |
The protein has a predicted MW of 20.1 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human FGF-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 1.22ug/ml determined by ELISA (QC Test). The affinity constant of 18.91 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22um filtered solution in 50mM Tris, 250mM NaCl (pH 7.5). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human FGF-7, His Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 18.91 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human FGF-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 1.22ug/ml determined by ELISA (QC Test). |
|
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Human FGF-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human FGF-7 is greater than 95% as determined by SEC-HPLC. |
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