Name: Human FGF-7/KGF Protein (KGF-HE101)
Brand: KactusBio
SKU: KGF-HE101-100UG
Price: 330.0 USD
Availability: InStock

Express System: E.coli
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human FGF-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 1.22ug/ml determined by ELISA (QC Test). The affinity constant of 18.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Cys32-Thr194

Catalog #KGF-HE101

Size

100UG

500UG

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Human FGF-7/KGF Protein (KGF-HE101) Summary

Product Description	Recombinant Human FGF-7/KGF Protein is expressed from E.coli with His tag at the N-Terminus.It contains Cys32-Thr194.
Product Background	The expression patterns of mRNAs encoding Fibroblast Growth Factor-7 (FGF-7) and its high affinity receptor suggested that FGF-7 signaling may play a role in regulating ureteric bud growth.t Results of these studies demonstrate that the developing ureteric bud and mature collecting system of FGF-7-null kidneys is markedly smaller than wild type. FGF-7 levels modulate the extent of ureteric bud growth during development and the number of nephrons that eventually form in the kidney.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human FGF-7/KGF Protein (KGF-HE101) Specifications/Details

Protein	FGF-7/KGF
Synonyms	FGF-7; HBGF-7; KGF; FGF7
Accession	P21781-1
Species	Human
Biotinylated	no
Amino Acid Range	Cys32-Thr194
Molecular Weight	The protein has a predicted MW of 20.1 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human FGF-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 1.22ug/ml determined by ELISA (QC Test). The affinity constant of 18.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM Tris, 250mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human FGF-7, His Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 18.91 nM as determined in SPR assay (Biacore T200).
	Immobilized Human FGF-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 1.22ug/ml determined by ELISA (QC Test).
	Human FGF-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human FGF-7 is greater than 95% as determined by SEC-HPLC.