Human FGF-7/KGF Protein (KGF-HE101)
Human FGF-7/KGF Protein (KGF-HE101)
  • Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FGF-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 1.22ug/ml determined by ELISA (QC Test). The affinity constant of 18.91 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Cys32-Thr194
  • Other Available Proteins:
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Catalog #KGF-HE101
Regular price $330.00
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Size
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Human FGF-7/KGF Protein (KGF-HE101) Summary

Product Description

Recombinant Human FGF-7/KGF Protein is expressed from E.coli with His tag at the N-Terminus.It contains Cys32-Thr194.

Product Background

The expression patterns of mRNAs encoding Fibroblast Growth Factor-7 (FGF-7) and its high affinity receptor suggested that FGF-7 signaling may play a role in regulating ureteric bud growth.t Results of these studies demonstrate that the developing ureteric bud and mature collecting system of FGF-7-null kidneys is markedly smaller than wild type. FGF-7 levels modulate the extent of ureteric bud growth during development and the number of nephrons that eventually form in the kidney.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human FGF-7/KGF Protein (KGF-HE101) Specifications/Details

Protein
FGF-7/KGF
Synonyms
FGF-7; HBGF-7; KGF; FGF7
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Cys32-Thr194
Molecular Weight
The protein has a predicted MW of 20.1 kDa same as Bis-Tris PAGE result.
Activity
Immobilized Human FGF-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 1.22ug/ml determined by ELISA (QC Test). The affinity constant of 18.91 nM as determined in SPR assay (Biacore T200).
Product Tag
N-His

Fluorophore

Unconjugated
Expression System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22um filtered solution in 50mM Tris, 250mM NaCl (pH 7.5).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human FGF-7, His Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 18.91 nM as determined in SPR assay (Biacore T200). Human FGF-7, His Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 18.91 nM as determined in SPR assay (Biacore T200).
Immobilized Human FGF-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 1.22ug/ml determined by ELISA (QC Test). Immobilized Human FGF-7, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 1.22ug/ml determined by ELISA (QC Test).
Human FGF-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human FGF-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human FGF-7 is greater than 95% as determined by SEC-HPLC. The purity of Human FGF-7 is greater than 95% as determined by SEC-HPLC.
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