Product Description |
Recombinant Human FGF basic (154aa) Protein is expressed from E.coli without tag.It contains Ala135-Ser288. |
Product Background |
FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. |
Product Category |
Recombinant Protein |
Target |
FGF basic (154aa) |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala135-Ser288 |
Molecular Weight |
The protein has a predicted MW of 17.12 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human FGF basic (154aa) at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
No Tag |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human FGF basic (154aa) at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). |
|
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Human FGF basic (154aa) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 0.1 - 0.5 ng/ml. |
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The purity of Human FGF basic (154aa) is greater than 95% as determined by SEC-HPLC. |
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