Name: Human FGF basic (154aa) Protein (FGF-HE002)
Brand: KactusBio
SKU: FGF-HE002-100UG
Price: 68.0 USD
Availability: InStock

Human FGF basic (154aa) Protein (FGF-HE002)

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human FGF basic (154aa) at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ala135-Ser288

Catalog #FGF-HE002

Size

100UG

500UG

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Product Description	Recombinant Human FGF basic (154aa) Protein is expressed from E.coli without tag.It contains Ala135-Ser288.
Product Background	FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.
Product Category	Recombinant Protein

Target	FGF basic (154aa)
Accession	P09038-4
Species	Human
Amino Acid Range	Ala135-Ser288
Molecular Weight	The protein has a predicted MW of 17.12 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human FGF basic (154aa) at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	No Tag
Express System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human FGF basic (154aa) at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test).
	Human FGF basic (154aa) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 0.1 - 0.5 ng/ml.
	The purity of Human FGF basic (154aa) is greater than 95% as determined by SEC-HPLC.

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