Human FGF basic Protein (FGF-HE001)
Catalog: FGF-HE001
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Product Description | Recombinant Human FGF basic Protein is expressed from E.coli without tag; It contains Pro143-Ser288. |
Product Background |
FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. |
Product Category |
Recombinant Protein |
Target |
FGF basic |
Synonyms | bFGF; FGF basic; FGF2; FGF-2; FGF2AS; GFG1; HBGF-2; NUDT6; Prostatropin; BFGF; FGFB; FGFβ; HBGF2 |
Accession | |
Species |
Human |
Amino Acid Range |
Pro143-Ser288 |
Molecular Weight | The protein has a predicted MW of 16.5 kDa same as Tris-Bis PAGE result. |
Activity |
Immobilized Human FGF basic at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 12.6 ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
No Tag |
Express System |
E.coli |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 0.02 EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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