Human FGF basic Protein (FGF-HE001)

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human FGF basic at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 12.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Pro143-Ser288

Catalog: FGF-HE001

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Product Description	Recombinant Human FGF basic Protein is expressed from E.coli without tag.It contains Pro143-Ser288.
Product Background	FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.
Product Category	Recombinant Protein

Target	FGF basic
Synonyms	bFGF; FGF basic; FGF2; FGF-2; FGF2AS; GFG1; HBGF-2; NUDT6; Prostatropin; BFGF; FGFB; FGFβ; HBGF2
Gene/Protein ID	P09038-4
Species	Human
Exact Sequence	Pro143-Ser288
Molecular Weight	The protein has a predicted MW of 16.5 kDa same as Tris-Bis PAGE result.
Activity	Immobilized Human FGF basic at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 12.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	No Tag
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human FGF basic at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 12.6ng/ml determined by ELISA (QC Test).
	Immobilized Human FGF basic, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIc), His Tag with the EC50 of 1.18ug/ml determined by ELISA.
	Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50-for this effect is 0.02 - 0.1 ng/ml (QC Test).
	Human FGF basic on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.