Human FGF basic Protein (FGF-HE001)

  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human FGF basic at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 12.6ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Pro143-Ser288

Catalog: FGF-HE001

$90.00
Size:

Order now get it on

Product Description Recombinant Human FGF basic Protein is expressed from E.coli without tag.It contains Pro143-Ser288.

Product Background

FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.

Product Category

Recombinant Protein

Target

FGF basic
Synonyms bFGF; FGF basic; FGF2; FGF-2; FGF2AS; GFG1; HBGF-2; NUDT6; Prostatropin; BFGF; FGFB; FGFβ; HBGF2

Gene/Protein ID

P09038-4

Species

Human

Exact Sequence

Pro143-Ser288
Molecular Weight The protein has a predicted MW of 16.5 kDa same as Tris-Bis PAGE result.

Activity

Immobilized Human FGF basic at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 12.6ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

No Tag

Express System

E.coli
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human FGF basic at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 12.6ng/ml determined by ELISA (QC Test).
Immobilized Human FGF basic, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIc), His Tag with the EC50 of 1.18ug/ml determined by ELISA.
Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50-for this effect is 0.02 - 0.1 ng/ml (QC Test).
Human FGF basic on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in