Sensorgram showing multi-concentration binding response of an analyte to immobilized ligand over time in an SPR assay.
Human FGF10 Protein (FGF-HE010)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 90% as determined by Bis-Tris PAGE
  • Activity: The affinity constant of 8.43 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln38-Ser208
Catalog #FGF-HE010
Regular price $264.00
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Human FGF10 Protein (FGF-HE010) Summary

Product Description

Recombinant Human FGF10 Protein is expressed from E.coli without tag.It contains Gln38-Ser208.

Product Background

Fibroblast growth factor 10 (FGF10) regulates multiple stages of structural lung morphogenesis, cellular differentiation, and the response to injury. As a driver of lung airway branching morphogenesis, FGF10 signaling defects during development lead to neonatal lung disease. Lung diseases impact patients across the lifespan, from infants in the first minutes of life through the aged population. Congenital abnormalities of lung structure can cause lung disease at birth or make adults more susceptible to chronic disease.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human FGF10 Protein (FGF-HE010) Specifications/Details

Protein
FGF10
Synonyms
FGF-10; KGF2
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln38-Ser208
Molecular Weight
The protein has a predicted MW of 19.3 kDa. The protein migrates to 25 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 8.43 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

No Tag
Expression System
E.coli
Purity
> 90% as determined by Bis-Tris PAGE
Endotoxin
Less than 0.1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human FGF10, No Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb), hFc Tag with an affinity constant of 8.43 nM as determined in SPR assay (Biacore T200). Human FGF10, No Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb), hFc Tag with an affinity constant of 8.43 nM as determined in SPR assay (Biacore T200).
Human FGF10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human FGF10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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