Product Description |
Recombinant Human FGF21 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains His29-Ser209. |
Product Background |
Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia. |
Product Category |
Recombinant Protein |
Target |
FGF21 |
Synonyms |
UNQ3115; PRO10196; FGF-21; FGF21 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
His29-Ser209 |
Molecular Weight |
The protein has a predicted MW of 20.2 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 20nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human FGF21, His Tag immobilized on CM5 Chip can bind Human Beta Klotho, His Tag with an affinity constant of 20nM as determined in a SPR assay (Biacore T200). |
|
|
Human FGF21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human FGF21 is greater than 95% as determined by SEC-HPLC. |
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