Binding kinetics sensorgram of a recombinant protein showing response units over time across multiple concentrations, used to evaluate affinity and interaction dynamics.
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: The affinity constant of 20nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: His29-Ser209
Catalog #FGF-HM121
Regular price $412.50
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Human FGF21 Protein (FGF-HM121) Summary

Product Description

Recombinant Human FGF21 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains His29-Ser209.

Product Background

Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human FGF21 Protein (FGF-HM121) Specifications/Details

Protein
FGF21
Synonyms
UNQ3115; PRO10196; FGF-21; FGF21
Accession
Species
Human
Biotinylated
no
Amino Acid Range
His29-Ser209
Molecular Weight
The protein has a predicted MW of 20.2 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 20nM as determined in SPR assay (Biacore T200).
Product Tag
N-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human FGF21, His Tag immobilized on CM5 Chip can bind Human Beta Klotho, His Tag with an affinity constant of 20nM as determined in a SPR assay (Biacore T200). Human FGF21, His Tag immobilized on CM5 Chip can bind Human Beta Klotho, His Tag with an affinity constant of 20nM as determined in a SPR assay (Biacore T200).
Human FGF21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human FGF21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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