Human FGF21 Protein (FGF-HM621)

  • Express System: HEK293
  • Product Tag: N-mFc-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FGF21 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 2.29ug/ml determined by ELISA (QC Test). The affinity constant of 0.54nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: His29-Ser209

Catalog: FGF-HM621

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Product Description Recombinant Human FGF21 Protein is expressed from HEK293 with mFc (IgG1) tag and Avi tag at the N-Terminus.It contains His29-Ser209.

Product Background

Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.

Product Category

Recombinant Protein

Target

FGF21
Synonyms UNQ3115; PRO10196; FGF-21; FGF21
Accession

Species

Human

Amino Acid Range

His29-Ser209
Molecular Weight The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human FGF21 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 2.29ug/ml determined by ELISA (QC Test). The affinity constant of 0.54nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-mFc-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200).
Immobilized Human FGF21 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 2.29ug/ml determined by ELISA (QC Test).
The purity of Human FGF21 is greater than 95% as determined by SEC-HPLC.
Human FGF21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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