Name: Human FGF21 Protein (FGF-HM621)
Brand: KactusBio
SKU: FGF-HM621-100UG
Price: 375.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-mFc-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human FGF21 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.75ug/ml determined by ELISA (QC Test). The affinity constant of 0.54nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: His29-Ser209

Catalog #FGF-HM621

Size

100UG

500UG

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Product Description	Recombinant Human FGF21 Protein is expressed from HEK293 with mFc (IgG1) tag and Avi tag at the N-Terminus.It contains His29-Ser209.
Product Background	Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.
Product Category	Recombinant Protein / Antibody discovery

Protein	FGF21
Synonyms	UNQ3115; PRO10196; FGF-21; FGF21
Accession	Q9NSA1-1
Species	Human
Biotinylated	no
Amino Acid Range	His29-Ser209
Molecular Weight	The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human FGF21 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.75ug/ml determined by ELISA (QC Test). The affinity constant of 0.54nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-mFc-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200).
	Immobilized Human FGF21 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.75ug/ml determined by ELISA (QC Test).
	Human FGF21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human FGF21 is greater than 95% as determined by SEC-HPLC.