Human FGF21 Protein (FGF-HM621)

  • Express System: Expi293
  • Product Tag: N-mFc-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FGF21 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 1.48μg/ml determined by ELISA. The affinity constant of 0.54nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: His29-Ser209

Catalog: FGF-HM621

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Product Description Recombinant Human FGF21 Protein is expressed from Expi293 with mFc tag and Avi tag at the N-terminal.It contains His29-Ser209.
Product Category Recombinant Protein
Target FGF21
Synonyms UNQ3115; PRO10196; FGF-21; FGF21
Gene/Protein ID Q9NSA1-1
Species Human
Exact Sequence His29-Ser209
Molecular Weight The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human FGF21 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 1.48μg/ml determined by ELISA. The affinity constant of 0.54nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-mFc-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200).
Immobilized Human FGF21 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 1.48μg/ml determined by ELISA.
The purity of Human FGF21 is greater than 95% as determined by SEC-HPLC.
Human FGF21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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