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Human FGFR1 alpha (IIIc) Protein (FGF-HM1RA) Summary
Product Description |
Recombinant Human FGFR1 alpha (IIIc) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Arg22-Glu374. |
Product Background |
Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding.FGFR1 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human FGFR1 alpha (IIIc) Protein (FGF-HM1RA) Specifications/Details
Protein |
FGFR1 alpha (IIIc) |
Synonyms |
FGF R1a; FGFR1 alpha |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg22-Glu374 |
Molecular Weight |
The protein has a predicted MW of 40.74 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human FGFR1 alpha (IIIc), His Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human FGF1, His Avi Tag with the EC50 of 98.7ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human FGFR1 alpha (IIIc), His Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human FGF1, His Avi Tag with the EC50 of 98.7ng/ml determined by ELISA. |
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Immobilized Human FGFR1 alpha (IIIc), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. |
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Human FGFR1 alpha (IIIc) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human FGFR1 alpha (IIIc) is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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