Regular price
$330.00
Order by 4pm ET for delivery on:
Human FGFR2 beta (IIIb) Protein (FGF-HM12D) Summary
Product Description |
Recombinant Human FGFR2 beta (IIIb) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Pro154-Leu358. |
Product Background |
Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha- isoform, or only IgII and IgIII, referred to as the beta- isoform. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Human FGFR2 beta (IIIb) Protein (FGF-HM12D) Specifications/Details
Protein |
FGFR2 beta (IIIb) |
Synonyms |
Fibroblast growth factor receptor 2;FGFR-2;KGFR;K-sam;Keratinocyte growth factor receptor;CD332;BEK;KSAM |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Pro154-Leu358 |
Molecular Weight |
The protein has a predicted MW of 24 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human FGFR2 beta (IIIb) at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. The affinity constant of 18.91 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human FGF-7, His Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 18.91 nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Human FGFR2 beta (IIIb) at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. |
|
|
Human FGFR2 beta (IIIb) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human FGFR2 beta (IIIb) is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
