Human FGFR2 beta (IIIb) Protein (FGF-HM12D)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FGFR2 beta (IIIb) at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test). The affinity constant of 18.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Pro154-Leu358

Catalog: FGF-HM12D

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Product Description Recombinant Human FGFR2 beta (IIIb) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Pro154-Leu358.

Product Background

Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha- isoform, or only IgII and IgIII, referred to as the beta- isoform.

Product Category

Recombinant Protein

Target

FGFR2 beta (IIIb)
Synonyms Fibroblast growth factor receptor 2;FGFR-2;KGFR;K-sam;Keratinocyte growth factor receptor;CD332;BEK;KSAM
Accession

Species

Human

Amino Acid Range

Pro154-Leu358
Molecular Weight The protein has a predicted MW of 24 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human FGFR2 beta (IIIb) at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test). The affinity constant of 18.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human FGF-7, His Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 18.91 nM as determined in SPR assay (Biacore T200).
Immobilized Human FGFR2 beta (IIIb) at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test).
The purity of Human FGFR2 beta (IIIb) is greater than 95% as determined by SEC-HPLC.
Human FGFR2 beta (IIIb) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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