Human FGFR2 beta (IIIb) Protein (FGR-HM2BB)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human FGFR2 beta (IIIb) , hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 5ng/ml determined by ELISA (QC Test). The affinity constant of 0.299 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Arg152-Glu378

Catalog: FGR-HM2BB

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Product Description	Recombinant Human FGFR2 beta (IIIb) Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Arg152-Glu378.
Product Category	Recombinant Protein

Target	FGFR2 beta (IIIb)
Synonyms	Fibroblast growth factor receptor 2；FGFR-2；KGFR；K-sam；Keratinocyte growth factor receptor；CD332；BEK；KSAM
Gene/Protein ID	P21802-3
Species	Human
Exact Sequence	Arg152-Glu378
Molecular Weight	The protein has a predicted MW of 52.2 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human FGFR2 beta (IIIb) , hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 5ng/ml determined by ELISA (QC Test). The affinity constant of 0.299 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human FGF10, No Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb) , hFc Tag with an affinity constant of 0.299 nM as determined in SPR assay (Biacore T200).
	Immobilized Human FGFR2 beta (IIIb) , hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 5ng/ml determined by ELISA (QC Test).
	The purity of Human FGFR2 beta (IIIb) is greater than 95% as determined by SEC-HPLC.
	Human FGFR2 beta (IIIb) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.