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Human FGFR2 beta (IIIb) Protein (FGR-HM2BB) Summary
Product Description |
Recombinant Human FGFR2 beta (IIIb) Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Arg152-Glu378. |
Product Background |
Four distinct genes encoding closely related FGF receptors, FGFR1-4, are known. All four genes for FGFRs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGFR1-3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGFR1 and 2 results in receptors containing all three Ig domains, referred to as the alpha- isoform, or only IgII and IgIII, referred to as the beta isoform. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Human FGFR2 beta (IIIb) Protein (FGR-HM2BB) Specifications/Details
Protein |
FGFR2 beta (IIIb) |
Synonyms |
Fibroblast growth factor receptor 2;FGFR-2;KGFR;K-sam;Keratinocyte growth factor receptor;CD332;BEK;KSAM |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg152-Glu378 |
Molecular Weight |
The protein has a predicted MW of 52.2 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human FGF-7, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 0.28 ug/ml determined by ELISA. The affinity constant of 8.43 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human FGF10, No Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb), hFc Tag with an affinity constant of 8.43 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human FGF-7, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 0.28 ug/ml determined by ELISA. |
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Immobilized Human FGF basic, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 33.0ng/ml determined by ELISA. |
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Immobilized Human FGFR2 beta (IIIb) , hFc Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 19.9 ng/ml determined by ELISA. |
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Human FGFR2 beta (IIIb) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of Human FGFR2 beta (IIIb) is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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