Non-biotinylated Human FGFR3 beta (IIIb) Protein (FGF-HM4BB) Summary
Product Description |
Recombinant Human FGFR3 beta (IIIb) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp127-Gly377. |
Product Background |
Four distinct genes encoding closely related FGF receptors, FGF R1-4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid‑box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain.FGFR3 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Non-biotinylated Human FGFR3 beta (IIIb) Protein (FGF-HM4BB) Specifications/Details
Protein |
FGFR3 bata (IIIb) |
Synonyms |
ACH; CD333; CEK; CEK2; EC 2.7.10; FGF R3; FGFR3; HSFGFR3EX; JTK4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp127-Gly377 |
Molecular Weight |
The protein has a predicted MW of 30.5 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human FGFR3 beta (IIIb), His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human FGFR3 beta (IIIb), His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA (QC Test). |
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Human FGFR3 beta (IIIb) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human FGFR3 beta (IIIb) is greater than 95% as determined by SEC-HPLC. |
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